黏彈性流體於剪流場下的微觀行為研究｜國立中央大學博碩士論文系統

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研究生：	徐敏恭 Min-kung Hsu
論文名稱：	黏彈性流體於剪流場下的微觀行為研究 The Microscopic Behaviors of Sheared Viscoelastic Fluids
指導教授：	阮文滔 Wen-tau Juan
口試委員:
學位類別：	碩士 Master
系所名稱：	理學院 - 生物物理研究所 Graduate Institute of Biophysics
畢業學年度：	96
語文別：	英文
論文頁數：	42
中文關鍵詞：	黏彈性流體、濃縮高分子溶液、單分子、剪力
外文關鍵詞：	shear, single molecule, concentrated polymer solution, viscoelastic fluids
相關次數：	點閱：10 下載：0
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牛頓流體和濃縮高分子溶液在剪力下的反應在單分子的層次被究，57%的蔗糖溶液做為牛頓流體而將λ-DNA 溶入TE 緩衝溶液中得到濃度5 ug/ul做為濃縮高分子溶液，Mn2+離子被加入濃縮高分子溶液中做為cross-linker. 螢光粒子被用來追蹤速度剖面圖，並以染色DNA 分子則用來觀察高分子微觀行為，剪力流場是由驅動一片在樣本之上的平板來產生。藉由螢光顯微技術，研究在牛頓流體和濃縮高分子溶液中單一高分子在剪力下的動態行為，在濃縮高分子溶液中速度剖面圖並非直線且DNA 分子的橢圓率也不是常數，當剪力停止之後濃縮高分子溶液表現出緩慢的舒張過程，由含有cross-linker 的濃縮高分子溶液中的單分子觀察並配合速度剖面圖和舒張過程，我們認為含有cross-linker 的高分子溶液為一具有堅固的三維連結網狀系統。

The responses of Newtonian fluid and concentrated polymer solutions under shear are studied at single molecule level. In this experiment, Newtonian fluids are 57% sucrose solutions and high concentration polymer solutions are prepared by dissolving λ‐DNA in TE buffer at 5 μg/μl concentration. The Mn2+ ions are added in the concentrated polymer solution to investigate the polymer solution at cross‐linked state. Fluorescence tracer particles are introduced in various flows; to measure the
velocity profiles. Dye labeled DNA molecules mixing with unlabeled ones are tracer molecules to observe the single molecule behaviors. The shear flow is generated by driving a plate on top of the fluid sample. Through the fluorescent microscopy, the dynamical behavior of individual molecule in the Newtonian fluid and concentrated
DNA solution under shear is investigated. The velocity profiles are nonlinear and the ellipse ratio of individual DNA molecules is not homogeneous in sheared concentrated polymer solution. The concentrated polymer solutions also show a slow relaxation process after shear. Base on the measured velocity profiles and the post‐shear relaxation process, we propose that, the cross‐linked concentrated polymer solution seems to be a strongly coupled 3D polymer network.

摘要 ......................................................................................................................................................... i
Abstract .................................................................................................................................................. ii
誌謝 ........................................................................................................................................................ iii
Content ................................................................................................................................................... iv
List of Figures .......................................................................................................................................... vi
Introduction ......................................................................................................................................... 1
Background .......................................................................................................................................... 4
1 Polymer solutions .......................................................................................................................... 4
1.1 Polymers dissolve in solutions ................................................................................................ 5
1.2 Polymer‐solvent phase diagram ............................................................................................. 6
1.3 Cross‐linked polymer solutions .............................................................................................. 8
2 Viscoelastic behaviors ................................................................................................................. 10
2.1 Viscoelasticity of material .................................................................................................... 10
2.2 Elastic behaviors versus viscoelastic behaviors .................................................................... 11
3 Polymer reptation ....................................................................................................................... 12
4 Single molecule studies in polymer science ................................................................................ 14
Experiment ........................................................................................................................................ 16
1 Fluorescence microscopy ............................................................................................................ 16
2 To prepare sample solutions ....................................................................................................... 17
2.1 Newtonian fluid .................................................................................................................... 17
2.2 Polymeric solution and cross‐linkers .................................................................................... 17
3 Experimental setup ...................................................................................................................... 19
3.1 To make shear stress ............................................................................................................ 19
3.2 Fluorescence microscopy observation ................................................................................. 21
Results and discussions ...................................................................................................................... 23
1 Sheared Newtonian fluid ............................................................................................................. 24
1.1 Velocity profiles of Newtonian fluid when shear ................................................................. 24
1.2 Single molecule observation in sheared Newtonian fluid .................................................... 27
2 Sheared High concentration DNA solution .................................................................................. 29
2.1 Velocity profile of polymeric solutions when shear ............................................................. 29
2.2 Single molecular behaviors of sheared concentrated solution ............................................ 32
2.3 Relaxation behavior of polymeric solution after shear ........................................................ 34
3 The microscopic behaviors of sheared Newtonian fluid and polymeric solutions ..................... 39
3.1 The velocity profiles ............................................................................................................. 39
3.2 The single molecular behaviors ............................................................................................ 39
Conclusions ........................................................................................................................................ 41
Bibliography .......................................................................................................................................... 43

                                

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