本研究以磺胺二甲嘧啶 (Sulfamethazine，SMZ) 為模板分子，四乙氧基矽烷 (Tetraethoxysilane，TEOS)為單體，經溶膠-凝膠程序與後處理程序製備分子拓印高分子(molecularly imprinted polymer, MIP)與無分子拓印高分子(non-molecularly imprinted polymer, NIP)。所討論的變數為(1)溶膠-凝膠程序之R值(H2O/TEOS) (2)後處理程序 (3)移動相pH值 (4)移動相組成，經溶膠-凝膠程序所得之產物因其網目架橋密度低、極性官能基多且須移除模板分子(SMZ)，須經後處理程序即萃取、HMDS修飾反應與進一步反應等步驟才能得到具有層析分離之MIP，故探討下列以下變數(1)HMDS修飾反應時間 (2)後處理程序的順序 (3)進一步反應時間 (4)鍛燒移除模板分子之溫度 (5) 移除模板分子方法即鍛燒與萃取比較，將製備之MIP作為層析靜置相，最後再以高效能液相層析儀(high performance liquid chromatographic method，HPLC) 測量SMZ與相似物磺胺甲噁唑 (Sulfamethoxazole，SMO)滯留時間進而算出選擇率(selectivity，α)與拓印因子(Imprinting factor，IF )，以及利用SMZ與SMO兩吸收峰的基部寬度進而計算出解析度(Resolution，Rs)與理論板數( Theoretical Plate Number，N )。
結果顯示溶膠-凝膠程序之R值會影響MIP之模板分子辨識位置，HMDS修飾反應時間、進一步反應的時間與移除模板分子之溫度會影響MIP及NIP之SMZ與SMO之滯留時間與層析分離之效果。萃取與鍛燒移除模板方法中，利用萃取所得之MIP其Rs與N高。移動相pH值接近模板分子的pKa值時(pH=7.4)及移動相含水量為90％時，有較高的α、IF、Rs與N。本法顯示先進行HMDS修飾反應60分鐘後在300℃反應2小時，最後利用甲醇萃取移除模板分子(SMZ)，移動相pH值及含水量比分別為7.4及90 ％ ，使MIP具有較高之α(4.03)、IF(2.19)、Rs(4.61)與N(361)

The synthesis of inorganic molecularly imprinted polymer (MIP) as a stationary phase of high performance liquid chromatography (HPLC), for the efficient determination of template (sulfamethazine, SMZ) and analogue (sulfamethoxazole, SMO) has been developed. MIP was prepared by reaction of tetraethoxysilane (TEOS) via sol-gel process and post treatment. The template was dissolved in acid aqueous solution and added into TEOS to form the precursor solution. The solution was adjusted the pH by adding ammonium hydroxide (NH4OH). There were three steps in post treatment , modification with HMDS, farther reaction, and extraction, to improve the crosslink density of MIP and remove template. The best recognizability of MIP was obtained by the following conditions: the ratio of water to TEOS (R=5), extraction (MeOH, 17hr), post treatment sequence (modification 1h, reaction 2h, extraction 17hr), the value of pH (pH= pka = 7.4) and composition of mobile phase (MeCN:H2O = 10:90).

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