在本論文中，我們提出小貓式自泵浦相位共軛反射鏡(Kitty-SPPCM)之延伸，對此相位共軛反射鏡作不同工作區域之探討，發現具有高通濾波之特性之自泵浦相位共軛反射鏡，並在實驗中發現一新型自泵浦相位共軛反射鏡，其共軛光產生機制類似橋式自泵浦相位共軛反射鏡，此實驗結果對於Kitty-SPPCM的應用將有更多向性的發展。接著，我們介紹利用Kitty-SPPCM提升數位全像架構之精準度，並嘗試利用數位全像術控制散射光散射行為，以空間光調製器調制讀取光的相位分布，雷射光通過老鼠皮膚組織仍後形成聚焦點。但受限於光學穿透能力仍有其極限，因此我們提出新穎的虛擬物鏡的概念，用以解決光穿透能力的限制，在厚仿生組織內產生聚焦點。最後，我們分別在散射片與老鼠皮膚所模擬的仿生模型中成功產生反向聚焦光。這項實驗的成功為厚生物體中顯微影像技術開啟了一扇窗，提供4pi技術應用於厚生物體之可能性，亦可被應用於厚生物體顯微檢測，以提升二倍頻及螢光訊號之強度。

Base on the Kitty self-pumped phase conjugate mirror (Kitty-SPPCM), a study for the incidence geometry to extend the accepted incidence position is presented and demonstrated in this thesis. When the incidence position is changed, a Kitty-SPPCM with high-pass filtering and a different SPPCM similar to the Bridge-SPPCM can be found. These conjugators extend the accepted incidence position range and angle, and it is helpful to apply the SPPCM in various new applications. The counter-directional Kitty-SPPCM is applied to optimize digital optical phase conjugation (DOPC) system alignment. With the precise DOPC system, the probing light can focus a spot through a mice’s skin. However, there is always a limit in optical penetration depth. A novel method is presented to focus light inside thick bio-tissue by a virtual objective lens, and a four-wave mixing system forming the virtual objective lens is demonstrated in experiment. This research is helpful to the 4-pi, second harmonic generation (SHG) and flourensence microscopy.

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