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研究生： 鄭峻翔
Chun-Shiang Cheng
論文名稱： 淡水河口之顆粒性有機碳、氮同位素及溶解性無機氮同位素之研究
A Study of Particulate Organic Carbon and Nitrogen and Dissolved Inorganic Nitrogen and Their Isotopic compositions in the Danshuei Estuary
指導教授： 劉康克
Kon-Kee Liu
高樹基
Shuh-Ji Kao
口試委員:
學位類別： 碩士
Master
系所名稱： 地球科學學院 - 水文與海洋科學研究所
Graduate Instittue of Hydrological and Oceanic Sciences
畢業學年度： 98
語文別： 中文
論文頁數： 187
中文關鍵詞： 同位素分化效應 、顆粒性有機物質 、氮循環
外文關鍵詞： Particulate organic matter, Isotope fractionation, Nitrogen cycle
相關次數： 點閱：14  下載：0 
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    • 淡水河位於臺灣北部，流域內工商業發達，人口聚居達五百萬以上。都市的廢水排入淡水河中，對河中懸浮物質與營養鹽有很大的影響。本研究乃利用碳、氮同位素作為示蹤劑，去探討有機物的來源。藉由分析顆粒性氮及溶解性無機氮之同位素組成，從兩者之關係，進而了解藻類吸收無機氮營養鹽之同位素分化作用。透過這些化學及同位素組成之分析，可以讓我們更了解淡水河系統中，有機物來源及生地化過程。本研究建立一個氨氮同位素標本製備方法:擴散法(Diffusion Method)，來量測淡水河的銨(d15N-NH4+)之氮同位素組成，對本研究極有助益。
    本研究進行了三次淡水河口採樣，在重陽橋利用兩個潮汐週期採得鹽度(0~16 psu)之水樣，可代表河口中段之狀況。主要目的是了解水體中營養鹽濃度的變化，以及與其它水文因子的關係，藉以探討影響淡水河顆粒性有機物的主要因子與生地化過程。
    淡水河之顆粒性氮同位素值在-17‰~+4‰之間，比世界其他河川的顆粒性氮之同位素值(-2‰~+20‰)來的輕很多。研究結果顯示，淡水河口之顆粒性有機物與葉綠素大多有良好之線性關係，顯示河口中藻類所產生之顆粒性有機物十分重要，推斷是藻類吸收污水中之營養鹽後生長出來，而藻類生長所需氮的主要來源是污水中的銨離子，在吸收過程中有強烈的同位素分化作用，顆粒性有機氮與銨離子之同位素差異可達-19‰。本研究首次證實Pennock等人(1996)在實驗室中培養矽藻所觀察到的受銨濃度影響之強烈同位素分化作用，在淡水河口的水體中的確會發生。
    在世界上許多研究過的河川中主要的氮物種是硝酸根，而淡水河口是以銨離子為主要的氮物種，由於藻類吸收銨離子之強烈分化作用，使得淡水河口中所量測到的顆粒性氮之同位素值，比世界其他河川所量測出之顆粒性氮之同位素值來的輕很多。
    由於淡水河河口之特殊環境，未來可對其氮循環及同位素分化作用作進一步之探討。

    The Danshuei River watershed in northern Taiwan is heavily industrialized and commercially developed with more than five million inhabitants. Anthropogenic wastes discharged to the Danshuei River strongly affect the concentrations of suspended organic matter and nutrients. In this study, we use carbon and nitrogen isotopic compositions to trace the origin of organic matter. By analyzing nitrogen isotopic compositions of particulate nitrogen (PN) and dissolved inorganic nitrogen (DIN), we may determine isotope fractionation during algal uptake of nitrogen. From chemical and isotopic compositions we are able to better understand biogeochemical process in the Danshuei River System. We also established the relatively simple diffusion method to measure d15N-NH4+ in the Danshuei Estuary.
    The results show that particulate nitrogen isotopic values (δ15NPN) in the Danshuei Estuary fall in the range from -17‰ to +4‰, which is lower than the values observed in other estuaries in the world. Since particulate organic matter (POM) and chlorophyll a (Chl-a) show good linear relationship, algae generated particulate organic matter (POM) should be important. The major source of nitrogen sustaining phytoplankton growth is ammonium (NH4+). The uptake process displays strong isotope fractionation with maximum δ15N shift reaching -19‰. Field observations of this study have corroborated the finding of Pennock et al., (1996) during culture of diatoms that ammonia uptake could be accompanied by large isotope fractionation.
    In most studied estuaries in the world, the major nitrogen species is nitrate (NO3-), whereas ammonium (NH4+) is the major nitrogen species in the Danshuei Estuary. Because algal uptake of ammonium (NH4+) has strong isotope fractionation, particulate nitrogen isotopic values (δ15NPN) in the Danshuei Estuary are unusually low.
    The distinctive biogeochemical features of the Danshuei Estuary warrant future studies on its nitrogen cycle and isotope fractionation processes.

    摘要 i Abstract iii 致謝 v 目錄 vii 表目錄 xi 圖目錄 xii 第一章 概說 1 1.1 氮循環 2 1.2 碳循環 4 1.3 穩定同位素 5 1.4 同位素分化作用 6 1.5 碳、氮同位素的變化 8 1.6 河口之定義 10 1.7 淡水河文獻回顧 11 1.8 研究目的 12 第二章 材料與方法 13 2.1 採樣方法 13 2.1.1 採樣地點與時間 13 2.1.2 觀測及採樣方式 14 2.1.3 污水處理廠標本取得與處理方式 15 2.2 分析方法 16 2.2.1 氨氮同位素分析：擴散法 16 2.2.2 固態法與溶液法 19 2.2.3 硝酸根氮之同位素：細菌法 21 2.2.3 顆粒性有機物(POM) 21 2.2.4 銨離子濃度測定方式 21 2.2.5 磷酸根濃度測定方式 25 2.2.6 亞硝酸根濃度測定方式 25 2.2.7 硝酸根濃度測定方式 26 2.2.8 矽酸根濃度測定方式 26 2.2.9 葉綠素a 27 2.2.10 鹼度 27 2.2.11 溶氧 31 2.3 同位素標本之測定 34 2.3.1 質譜儀 35 2.3.2 氮樣本分析流程 35 第三章 氨氮同位素標本製備之測試 37 3.1 數據處理步驟 37 3.1.1 最小平方直線作法(Derivation of a least-squares line) 37 3.1.2 測試樣本之數據處理方法 39 3.2 實驗之設計 41 3.3 分析結果 41 3.3.1 擴散法 41 3.3.2 固態法 43 3.3.3 溶液法 45 3.4 討論 46 3.5 小結 47 第四章 淡水河 49 4.1 淡水河系簡介 49 4.2 樣品採集時間與地點 51 4.3 結果 51 4.3.1 水位與鹽度 51 4.3.2 硝酸根(NO3-) 52 4.3.3 亞硝酸根(NO2-) 52 4.3.4 銨(NH4+) 52 4.3.5 磷酸根(PO43-) 53 4.3.6 矽酸根(SiO42-) 53 4.3.7 葉綠素(Chl-a) 53 4.3.8 溶氧(DO) 54 4.3.9 鹼度與總溶解性二氧化碳(Alk&TCO2) 55 4.3.10 顆粒性有機碳(POC)、顆粒性氮(PN)之濃度與同位素 55 4.3.11 污水處理廠採樣結果 56 4.4 討論 57 4.4.1 淡水河口營養鹽濃度變化探討 57 4.4.2 淡水河口溶氧濃度之探討 58 4.4.3 淡水河口之生地化作用 60 4.4.3.1 顆粒性有機物之變化 60 4.4.3.2 顆粒性氮與溶解性無機氮之同位素關係 61 4.4.3.3 顆粒性有機碳氮同位素組成與來源 61 4.4.3.4 藻類吸收銨之同位素分化作用 63 4.5 小結 65 第五章 結論與建議 67 5.1 結論 67 5.2 建議 69 英文參考文獻 70 中文參考文獻 75 附錄一 磷酸根濃度分析 139 附錄二 亞硝酸根濃度分析 143 附錄三 硝酸根濃度分析 146 附錄四 矽酸根濃度分析 149 附錄五 葉綠素a濃度分析 153 附錄六 野外採樣標本資訊及現場量測水文參數之原始數據 157 附錄七 野外採樣標本之營養鹽濃度之原始數據 161 附錄八 野外採樣標本之pH值、溶氧、鹼度及總溶解二氧化碳之原始數據 165 附錄九 野外採樣標本之顆粒有機碳氮濃度與同位素之原始數據 169 附錄十 2009年2月採樣，水位計連續觀測之資料 171 附錄十一 2009年2月採樣，CTD連續觀測之資料 176 附錄十二 2009年7月採樣，水位計連續觀測之資料 184

    Ahad, J.M.E., Ganeshram, R.S., Spencer, R.G.M., Uher, G., Upstill-Goddard, R.C., Cowie, G.L., 2006. Evaluating the sources and fate of anthropogenic dissolved inorganic nitrogen (DIN) in two contrasting North Sea estuaries. Science of the Total Environment 372, 317-333.
    Ashkenas, L.R., Johnson, S.L., Gregory, S.V., Tank, J.L., Wollhein, W.M., 2004. A stable isotope tracer study of nitrogen uptake and transformation in an old-growth forest stream. Ecology 85, 1725-1739.
    Barrie, A., Prosser, S.J., 1996. Automated analysis of light-element stable isotopes by isotope ratio mass spectrometer. Marcel Dekker, Inc, New York., 1-46 pp.
    Bigeleisen, J., 1949. The relative reaction velocities of isotopes molecules. J. Chem. Phys 17, 675-678.
    Biscaye, P.E., Flagg, C.N., Falkowski, P.G., 1994. The shelf-edge exchanges processes experiment, SEEP-II: an introduction to hypotheses, results and conclusions. Deep-Sea Research 41, 231-252.
    Cifuentes, L.A., Fogel, M.L., Pennock, J.R., Sharp, J.H., 1989. Seasonal variations in the stable nitrogen isotope ratio of ammonium in the Delaware estuary. Geochim. Cosmochim. Acta 53, 2713-2721.
    Clark, I.a.P.F., 2000. Isotope ratio mass spectrometry. Lewis Publishers, Boca Raton, Florida, 13-16 pp.
    Deines, P., 1980. The isotopic composition of reduced organic carbon. In: Fritz, P., Fontes, J.C. (Eds.), Handbook of environmental isotope geochemistry. Elservier Scientific, pp. 323-347.
    Duce, R.A., LaRoche, J., Altieri, K., Arrigo, K.R., Baker, A.R., Capone, D.G., Cornell, S., Dentener, F., Galloway, J., Ganeshram, R.S., Geider, R.J., Jickells, T., Kuypers, M.M., Langlois, R., Liss, P.S., Liu, S.M., Middelburg, J.J., Moore, C.M., Nickovic, S., Oschlies, A., Pedersen, T., Prospero, J., Schlitzer, R., Seitzinger, S., Sorensen, L.L., Uematsu, M., Ulloa, O., Voss, M., Ward, B., Zamora, L., 2008. Impacts of atmospheric anthropogenic nitrogen on the open ocean. Science 320, 893-897
    Fabbricino, M., Petta, L., 2007. Drinking water denitrification in membrane bioreactor/membrane contactor systems. Desalination 210, 163-174
    Fairbridge, R., 1980. The estuary: its definition and geodynamic cycle. John Wiley & Sons New York, 1-35 pp.
    Fry, B., Sherr, E.B., 1984. 13C measurements as indicators of carbon flow in marine and freshwater ecosystem. Contrib. Mar. Sci 27, 13-47.
    Galloway, J.N., Schlesinger, W.H., Levy, H., Michaels, A., Schnoor, J.L., 1995. Nitrogen Fixation: Anthropogenic Enhancement Environme- ntal Response. Glob Biogeochem Cycles 9, 235-252.
    Goolsby, D.A., Battaglin, W.A., Aulenbach, B.T., Hooper, R.P., 2000. Nitrogen Flux and Sources in the Mississippi River Basin. The Science of the Total Environment 248, 75-86.
    Hayes, J.M., 1993. Factors controlling 13C contents of sedimentary organic compounds: Principles and evidence. Marine Geology 113, 111-125.
    Hoefs, J., 1973. Stable Isotope Geochemistry. Springer, New York, 140 pp.
    Holmes, R.M., McClelland, J.W., Sigman, D.M., Fry, B., Peterson, B.J., 1998. Measuring 15N–NH4+ in marine, estuarine and fresh waters: An adaptation of the ammonia diffusion method for samples with low ammonium concentrations. Marine Chemistry 60, 235–243.
    Huang, W.R., Jones, W.K., 2001. Characteristics of long-term freshwater transport in Apalachicola Bay. Journal of the American Water Resources Association 37, 605-615.
    Junk, G., Svec, H.J., 1958. The absolute abundance of nitrogen isotopes in the atmosphere and compressed gas from various sources. Geochim. Cosmochim. Acta 14, 234-243.
    Lehninger, A.L., 1975. Biochemistry. Worth, New York, 1104 pp.
    Liu, K.K., 1979. Geochemistry of Inorganic Nitrogen Compounds in Two Marine Environments: The Santa Barbara Basin and the Ocean off Peru University of California Los Angeles, pp. 354.
    Liu, K.K., Kaplan, I.R., 1989. Eastern tropical Pacific as a source of 15N-enriched nitrate in seawater off southern California. Limn- ol.Oceanogr 34, 820-830.
    Liu, K.K., Tang, T.Y., Gong, G.C., Chen, L.Y., Shiah, F.K., 2000, a. Cross-shelf and along-shelf nutrient fluxes derived from flow fields and chemical hydrography observed in the southern East China Sea off northern Taiwan. Cont. Shelf Res 20(4-5), 493-253.
    Liu, K.K., Atkinson, L., Chen, C.T.A., Gao, S., Hall, J., Macdonald, R.W., McManus, L.T., Quinones, R., 2000, b. Exploring continental margin carbon fluxes on a global scale. Eos, Transactions, American Geophysical Union 81, 641-644.
    Liu, K.K., Kao, S.J., Wen, L.S., Chen, K.L., 2007. Carbon and nitrogen isotopic compositions of particulate organic matter and biogeo- chemical processes in the eutrophic Danshuei Estuary in northern Taiwan. Science of the Total Environment 382, 103–120.
    Liu, K.K., Seitzinger, S., Mayorga, E., Harrison, J., Ittekkot, V., 2008. Fluxes of nutrients and selected organic pollutants carried by rivers. SCOPE 70. Island Press, Washington D.C., 141-167 pp.
    Liu, K.K., Atkinson, L., Quiñones, R., Talaue-McManus, L., 2010. Biogeochemistry of continental margins in a global context. In: Liu, K.K., Atkinson, L., Quiñones, R., Talaue-McManus, L. (Eds.), Carbon and Nutrient Fluxes in Continental Margins: A Global Synthesis. IGBP Book Series. Springer, Berlin, pp. 3-24.
    Mackenzie, F.T., Ver, L.M., Sabine, C., Lane, M., Lerman, A., 1993. C, N, P, S global biogeochemical cycles and modeling of global change. In: Wollast, R., Mackenzie, F.T., Chou , L. (Eds.), Interactions of C, N, P and S Biogeochemical cycles and global change. Springer-Verlag, pp. 1-62.
    Mariotti, A., 1983. Atmospheric nitrogen is a reliable standard for natural 15N abundance. Nature 303, 685-687.
    Meybeck, M., 1982. Carbon, nitrogen, and phosphorus transport by world rivers. American Journal of Science 282, 401-450.
    Morel, F., M. M., Hering, J.G., 1983. Principles and Applications of Aquatic Chemistry. John Wiley and Sons, inc, New York.
    O''Leary, M.H., 1981. Carbon isotope fractionation in plants. Phytochemistry 20, 553-567.
    Pai, S.C., Yang, C.C., Riley, J.P., 1990. Formation kinetics of the pink azo dye in the determination of nitrite in natural waters. Anal. Chim. Acta 232, 245-349.
    Pai, S.C., Tsau, Y.J., Yang, T.I., 2001. pH and buffering capacity problems involved in the determination of ammonia in saline water using the indophenol blue spectrophotometric method. Analytica Chimica Acta 434, 209-216.
    Pennock, J.R., Velinsky, D.J., Ludlam, J.M., Sharp, J.H., Fogel, M.L., 1996. Isotopic fractionation of ammonium and nitrate during uptake by Skeletonema costatum: Implications for delta N-15 dynamics under bloom conditions. Limnol. Oceanogr 41, 451-459.
    Peters, K.E., Sweeney, R.E., Kaplan, I.R., 1978. Correlation of carbon and nitrogen stable isotope ratios in sedimentary organic matter. Limnol. Oceanogr 23, 598-604.
    Pritchard, D.W., 1967. What is an estuary: Physical Viewpoint. Washington, D. C. : Estuaries, 3-5.
    Rowe, G.T., Smith, S., Falkowski, P., Whitledge, T., Theroux, R., Phoel, W., Ducklow, H., 1986. Do continental shelves export organic matter? . Nature 324, 559-561.
    Sakett, W.M., 1989. Stable carbon isotope studies on organic matter in the marine environment. In: Fritz, P., Fontes, J.C. (Eds.), Handbook of environmental isotope geochemistry. Elsevier, pp. 139-170.
    Schlesinger, W.H., 1991. Biogeochemistry-an analysis of global change. Academic press.
    Sheltz, D.J., Calder, J.A., 1976. Organic carbon 13C/12C variations in the estuarine sediments. Geochim. Cosmochim. Acta 40, 381-385.
    Siegenthaler, U., Sarmiento, J.L., 1993. Atmospheric carbon dioxide and the ocean. Nature 365, 119-125.
    Sigman, D.M., Altabet, M.A., Michener, R., McCorkle, D.C., Fry, B., Holmes, R.M., 1997. Natural abundance-level measurement of the nitrogen isotopic composition of oceanic nitrate: an adaptation of the ammonia diffusion method. Marine Chemistry 57, 227-242.
    Sigman, D.M., Casciotti, K.L., Andreani, M., Barford, C., Galanter, M., Bohlke, J.K., 2001. A Bacterial Method for the Nitrogen Isotopic Analysis of Nitrate in Seawater and Freshwater. Anal. Chem 73, 4145-4153.
    Smith, B.N., Epstein, S., 1971. Two categories of 13C/12C ratios for higher plants. Plant Physoil 47, 380-384.
    Smith, S.V., Mackenzie, F.T., 1987. The ocean as a net heterotrophic system: Implications from the carbon biogeochemical cycle. Global Biogeochemical Cycles 1, 187-198.
    Sweeney, R.E., Liu, K.K., Kaplan, I.R., 1978. Organic nitrogen isotopes and their uses in determining the source of sedimentary nitrogen. In: Robinson, B.W. (Ed.), Stable Isotopes in the Earth Science. DSIR, New Zealand, pp. 9-26.
    Tieszen, L.L., Boutton, T.W., 1988. Stable isotopes in terrestrial ecosystem research. Springer, Berlin, 167-195 pp.
    Vitousek, P.M., Aber, J.D., Howarth, R.W., Likens, G.E., Matson, P.A., Schindler, D.W., Schlesinger, W.H., Tilman, D.G., 1997. Human Alteration of the Global Nitrogen Cycle: Sources and Consequences. Ecological Applications 7, 37-750.
    Vitousek, P.M., Mooney, H.A., Lubchenco, J., Melillo, J.M., 1997. Human Domination of Earth''s Ecosystems. Science 277, 494-499.
    Wada, E., Kadonaga, T., Matsuo, S., 1975. 15N in nitrogen of naturally occurring substances and global assessment of denitrification from isotopic viewpoint. Geochem. J 9, 139-148.
    Wada, E., 1980. Nitrogen isotope fractionation and its significance in biogeochemical processes occurring in marine environments. In: Goldberg, E., Horible, Y., Saruhashi, K. (Eds.), Isotope Marine Chemistry. Uchida-Rokakuho, Tokyo, pp. 375-398.
    Wada, E., Minagawa, M., Mizutani, H., Tsuji, T., Imaizumi, R., Karasawa, K., 1987. Biogeochemical studies on the transport of organic matter along the Otsuchi River watershed, Japan. Estuarine Coastal Shelf Sci. 25, 321-336.
    Wada, E., Hattori, A., 1991. Nitrogen in the sea: forms, abundances, and rate processes. CRC Press, Florida.
    Walsh, J.J., 1991. Importance of continental margins in the marine biogeochemical cycling of carbon and nitrogen. Nature 350, 53-55.
    Welschemeyer, N.A., 1994. Fluorometric analysis of chlorophyll a in the presence of chlorophyll b and phaeopigments. Limnology & Oceanography 39, 1985–1992.
    Wen, L.S., Jiann, K.T., Liu, K.K., 2008. Seasonal variation and flux of dissolved nutrients in the Danshuei Estuary, Taiwan: A hypoxic subtropical mountain river. Estuarine, Coastal and Shelf Science 78, 694–704.
    Zhang, J., 1996. Nutrient elements in large Chinese Estuaries. ContinentalShelf Research 16, 1023-1045.
    龔國慶, 1992. 台灣東北海域黑潮鋒面水文化學之研究. 國立台灣大學海洋科學研究所博士論文, 456.
    陳鎮東, 1994. 海洋化學 國立編譯館 茂昌圖書有限公司, 229-260.
    白書禎, 郭廷瑜, 鐘仕偉, 蘇宗德, 1998. 疊氮修正希巴辣光度測氧法及其在環境監測上應用. 化學(中國化學會) 56, 173-185.
    孫毓璋, 彭竟凱, 2001. 大漢溪流域水體環境中重金屬及營養鹽分佈的探討. 台灣海洋學刊, 39、105-120.
    陳冠倫, 2001. 水體中懸浮顆粒有機碳、氮同位素之研究：淡水河及東海南部. 國立台灣大學海洋研究所碩士論文, pp. 98.
    謝蕙蓮, 2001. 由底棲群聚看淡水河河口生態品質. 台灣海洋學刊, 39、121-134.
    彭明德, 2001. 淡水河之硝化現象模擬. 國立臺灣大學土木工程學研究所碩士論文 pp. 89.
    黃金山, 2001. 淡水河流域防洪計畫. 台灣海洋學刊, A24-A42.
    方天熹, 2001. 溶解態鋁在淡水河河口及近岸海域之分布. 台灣海洋學刊, 39、93-104.
    黃蔚人, 2003. 淡水河系中上游河水中氮物種之時空變化. 國立台灣大學海洋研究所碩士論文, pp. 153.
    彭宗仁, 劉滄棽, 林幸助, 2006. 穩定同位素在農業及生態環境上之應用. 台灣農業研究, 55-90.
    莊竣皓, 2006. 淡水河流域鹼度、酸鹼值與主要離子之時空變化. 國立中央大學水文科學研究所碩士論文, pp. 190.
    中華民國國家標準檢驗法, 2007. 深層海水檢驗法－磷酸鹽之測定. CNS 15091-15012, N 17001-15012.
    中華民國國家標準檢驗法, 2007. 深層海水檢驗法－亞硝酸鹽之測定. CNS 15091-15015, N 17001-15015.
    中華民國國家標準檢驗法, 2007. 深層海水檢驗法－矽酸鹽之測定. CNS 15091-15013, N 17001-15013.
    中華民國國家標準檢驗法, 2008. 深層海水檢驗法－氨之測定. CNS 15091-15029, N 17001-15029.
    中華民國國家標準檢驗法, 2008. 深層海水檢驗法－葉綠素a之測定. CNS 15091-15030, N 17001-15030.

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