簡易檢索 / 詳目顯示
| 研究生： |
涂閔忠 Min-Zhong Tu |
|---|---|
| 論文名稱： |
圓錐平板型生物反應器脈動式流場研究 Pulsatile Flow In A Cone-And-Plate Bioreactor |
| 指導教授： |
鍾志昂
Chih-Ang Chung |
| 口試委員: | |
| 學位類別： |
碩士 Master |
| 系所名稱： |
工學院 - 機械工程學系 Department of Mechanical Engineering |
| 畢業學年度： | 92 |
| 語文別： | 中文 |
| 論文頁數： | 70 |
| 中文關鍵詞： | 脈動式 、主流場 、剪應力 、圓錐平板型生物反應器 |
| 外文關鍵詞： | pulsatile, cone-and-plate bioreactor, primary flow, shear stress |
| 相關次數： | 點閱：9 下載：0 |
| 分享至: |
| 查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報 |
摘 要
生物反應器（bioreactor）為一種體外裝置，它可以提供適當的生化和機械刺激（如流體剪應力、拉伸、彎曲和壓縮等）的環境以促進細胞的生長及組織的變異。
本篇論文主要是在研究錐板型生物反應器內，流場剪應力的分佈情形。之前學者對於錐板型裝置的研究，均是考量圓錐是作單一方向性的旋轉。然而在真實的情況下，由於心臟的收縮與舒張會使得血液的流動為脈動（pulsatile）的形式，因此我們設定流場的流動為脈動式的。並且考慮此裝置具有邊界，研究邊界效應以及圓錐轉動頻率對於流場剪應力的影響。
Abstract
Bioreactor is an in-vitro apparatus that can impart appropriate biochemical and mechanical environments to promote cell growth and tissue differentiation. Among the mechanical stimulation apparatus for the cell and tissue cultures, the cone-and-plate system has been applied to provide shear stresses for such as the endothelial and cartilage responses studies. Most of the previous studies considered the cone rotated steadily to generate steady-state shear stimulus. In this paper we consider the flow field is pulsatile. Contemplating two parameters which regard the shear stress, one is the side wall effects and the other is related to the frequency of the cone, we set up the formulation for the shear stress in the cone-and- plate system.
參考文獻
1. Berger, S. A. , Goldsmith, W. and Lewis, E. R. ,“Introduction to bioengineering,”Oxford（1996）.
2. Brown, T. D. ,“Techniques for mechanical stimulation of cells in vitro：a review,”J. Biomech. 33, 3-14（2000）.
3. Bussolari, S. R. ,Dewey, C. F. and Gimbrone, M. A. ,“Apparatus for subjecting living cells to fluid shear stress,”Rev. Sci. Instrum. 53,1851-1854（1982）.
4. Chiu, J. J. , Chen, L. J. , Chen, C. N. , Lee, P. L. and Lee, C. I“A model for studying the effect of shear stress on interaction between vascular endothelial cells and smooth muscle cells,”J. Biomech. 37, 531-539（2004）.
5. Cox, D. B.“Radial flow in the cone-plate viscometer,”Nature 193, 670（1962）.
6. Dewey, C. F. , Bussolari, S. R. , Gimbrone, M. A. and Davies, P. F.“The dynamic respone of vascular endothelial cells to fluid shear stress,”J. Biomech. Eng. 103, 177-185（1981）.
7. Dewey, C. F.“Effects of fluid flow on living vascular cells,”J. Biomech. Eng. 106, 31-35（1984）.
8. Einav, S. , Dewey, C. F. and Hartenbaum, H.“Cone-and-plate apparatus：a compact system for studying well-characterized turbulent flow fields,”Experiments in Fluids 16, 196-202（1994）.
9. Hochleitner, B. W. , Hochleitner, E. O. , Obrist, P. , Eberl, T. , Amberger, A. , Xu, Q. , Margreiter, R. and Wick, G.“Fluid shear stress induced heat shock protein 60 expression in endothelial cells in vitro and in vivo,”Arterioscler Thromb Vasc. Biol. 20, 617（2000）.
10. Koslow, A. R. , Stromberg, R. R. , Friedman, L. I. , Lutz, R. J. , Hilbert, S. L. and Schuster, P.“A flow sysKtem for the study of shear forces upon cultured endothelial cells,”Transactions of the ASME 108, 338-341（1986）.
11. Malek, M. and Izumo, S.“Control of endothelial cell gene expression by flow,”J. Biomech. 28, 1515-1528（1995）.
12. Sdougos, H. P. , Bussolari, S. R. and Dewey, C. F.“Secondary flow and turbulence in a cone-and-plate device,”J. Fluid Mech. 138, 379-404（1984）.
13. Viggers, R. F. , Wechezak, A. R. and Sauvage, L. R.“An apparatus to study the response of cultured endothelium to shear stress,”Transactions of the ASME 108, 332-337（1986）.
14. White, F. M.“Viscous fluid flow,”second edition, McGraw-Hill（1991）.
