表面增強拉曼散射( SERS )作為是非破壞性光學檢測，經常被使用檢測各項生物樣本(吡啶、毒品、農藥、蛋白質、食品添加物、DNA和RNA)，本實驗選用非結構性的多孔矽作為固態基底，並在其上方沉積銀作為拉曼訊號增強來源，而實驗內容並非側重於單分子檢測或是銀結構的影響，而是著重於實際檢測與實用便利性，從實驗結果可以得知多孔矽由於劇烈的表面起伏和電負度為負的分子鍵尾，而產生疏水性的物理特性(表面能低)，和因沉積銀表面物理特性電負度為負轉正成為親水性(表面能高)，可透過”液珠沉積法”達成圖案化親疏水性的銀沉積點，其兼具多孔矽利於檢測樣本聚集且有仍然保持強拉曼訊號增強，除此之外，整體試片製作成本低廉且不需貴重儀器才能製作，只需在一般無塵室即可製作，除了上述優勢之外，其試片於實際樣本檢測中，由於樣本液滴因被限制其檢測樣本也被侷限於銀沉積點中，並不會樣本被浪費而造成靈敏度下降的問題，又因表面能差異樣本液滴自動對位於銀沉積點，更加便利於實際檢測過程，簡化很多於實驗過程中的流程，隨著液滴因被限制形成液滴透鏡，提供另一種方式可快速檢測的方式，不需要等待樣本液滴完全乾掉就可以檢測。

Surface Enhanced Raman Scattering (SERS) is used as a non-destructive optical detection. It is often used to detect various biological samples including pyridine, drugs, pesticides, proteins, food additives, DNA or RNA. In this experiment, non-structural porous silicon (PS) was used as the solid SERS substrate, and silver nanoparticles deposited on the PS surface was used as a Raman signal enhancement source. From the experimental results, it can be seen that PS has a strong surface undulation and negative electronegativity of the molecular bonding tails. Therefore, the physical properties of hydrophobicity (low surface energy) are generated as described above. The physical properties of the deposited silver surface become hydrophilic (high surface energy) as the electronegativity turns negative to positive. The patterned hydrophobic silver deposition point can be achieved by the droplet deposition method. The PS facilitates the detection of sample aggregation and still maintains strong Raman signal enhancement. In addition to that the total specimen production cost is low and does not require expensive equipment to produce, and can be produced in a normal clean room. In addition to the above advantages, the actual sample are limited by the silver deposition point of the sample droplets so that the sample is not wasted and the sensitivity is reduced. Because of the surface energy difference, the sample droplets are self-align at the silver deposition point, which facilitates the actual detection process and simplifies many processes in the experiment. As the droplet is confined, a droplet lens is formed. Provide another way to quickly detect.

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