| 研究生: |
蘇啟仁 Chi-Jen Su |
|---|---|
| 論文名稱: |
以Z曲線分析法探索人類基因體之辨識 |
| 指導教授: |
李弘謙
Hoong-Chien Lee |
| 口試委員: | |
| 學位類別: |
碩士 Master |
| 系所名稱: |
理學院 - 物理學系 Department of Physics |
| 畢業學年度: | 90 |
| 語文別: | 中文 |
| 論文頁數: | 53 |
| 中文關鍵詞: | Z曲線 |
| 外文關鍵詞: | Z - Curve |
| 相關次數: | 點閱:14 下載:0 |
| 分享至: |
| 查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報 |
摘要
目前對基因的分析方法有好幾種,而我們使用的方法是根據
Chun-Ting Zhang 所提出的Z 曲線分析法,這個方法是用多變數分析法作
分析,試著用對猜測基因部份作分析。
事實上在基因的判定原則上還是由實驗結果來決定,不過由於實
驗的成本相當的高,且費時,所以由Chun-Ting Zhang 所提出的Z 曲線分
析法可作初步的辨讀,並作探討。
我們對人類的24條染色體(包括2條性染色體)的mRNA部份作分
析,由於人類是真核生物,在轉錄轉譯蛋白質摺疊, 在
這過程中會將內碼子去除再各別將外碼子連接成mRNA,所形成的序
列就是我們要分析的序列。
參考文獻
[1] International human genome sequencing consortium, 2001. Initial sequencing and analysis of the human genome. Nature, 409, 824-955.
[2] ftp.ncbi.nih.gov/genbomk/genomes/H_sapiens/
[3] B. Lewin, “Gene”, 7th edition, (OXFORD, 2000).
[4] Peter H. Raven and George B. Johnson “Biolgy”, 5th edition (McGraw-Hill, Inc., 1999).
[5] Neil A. Campbell , Jane B. Reece and Lawrence G. Mitchell, “Biology”, 5th edition (Benjamin/Cummings, 1999).
[6] B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts, J. D. Watson,
‘‘Molecular Biology of The Cell’’, (Garland, New York, 1994).
[7] Zhang, C.-T. & Chou, K.-C. (1994) A graphic approach to analyzing
codon usage in 1562 Escherichia coli protein coding sequences. J. Mol. Biol. 238, 1-8.
[8] Chun-Ting Zhang and Ju Wang (2000) Recognition of protein coding genes in the yeast genome at better than 95% accuracy based on the Z curve. Nucleic Acids Res., 28, 2804-2814.
[9] Ju Wang and Chun-ting Zhang (2001) Identification of protein-coding genes in the genome of Vibrio cholerae with more than 98% accuracy using occurrence frequencies of single nucleotides. Eur. J. Biochem. 268, 4261-4286.
[10] Cornish-Bowden, A. (1985) Nucleic Acids Res., 13, 3021-3030.
[11] Reginald H. Garrett and Charles M. Grisham, “Biochemistry”, 2th edition, (HARCOURT Inc.,1999).
[12] Frederick R. Blattner, Guy Plunkett III,(1997) The Complete Genome Sequence of Escherichia coli K-12. Science. 277, 1453-1462.
[13] Nicole T. Perna, Guy Plunkett III,(2001) Genome sequence of enterohaemorrhagic Escherichia coli O157:H7. Nature. 409, 529-533.
[14] Chun-Ting Zhang and Kuo-Chen (1994) A Graphic Approach to Analyzing Codon Usage in 1562 Escherichia coli Protein Coding Sequences. J. Mol. Biol. 238, 1-8.
[15] Mardia, K.V. ,Kent, J. and Bibby, J.M.(1979) Multivariate Analysis. Academic Press, London, UK.
[16] Chris Burge and Samuel Karlin., J. Mol. Biol (1997) 268,78-94.
[17] Moises Burset and Roderic Guigo, Genom
[18] John B. Hogenesch, Keith A. Ching, Cell (2001) 106, 413-415.