裂褶菌多醣能有效提高生物體的免疫機能，活化老鼠體內巨噬細胞的吞噬能力，以及提升對小白鼠Sarcoma 180的腫瘤抑制率，可與西藥搭配治療用於幫助增強癌症病人的免疫系統以及抵抗腫瘤的能力，因此具有相當潛力被開發為未來醫療用品或作為近年來國內頗熱門的保健食品，視為未來的明星產品。
為了增加裂褶菌多醣的產量，本研究以液態培養發酵的方式以期增加多醣產量，然而影響發酵的環境因子很多，其中本實驗選擇以檸檬酸為探討變因，其原因來自曾有文獻指出(Jana et al., 1995 )添加檸檬酸於微生物的發酵環境能提高菌體耗氧速率以促進菌體產生大量ATP以供產物多醣生成所需之能量來源，且有關檸檬酸對真菌發酵的探討研究不多，所以本研究將以檸檬酸探討其對裂褶菌菌絲生長與多醣生成所造成的影響， 同時並引用數學模式來計算不同系統所對應的動力學參數值，試著從參數裡推測檸檬酸與裂褶菌代謝機制的關係。
本研究分別以搖瓶振盪及小型批式發酵槽的培養方式，採用之菌株為裂褶菌Schizophyllum commune ATCC 38548進行培養，以討論檸檬酸對菌絲生長及其多醣體生成的影響。
實驗結果分為兩大部分，第一部份為搖瓶振盪的結果，首先為篩選碳源部份，本實驗以單位菌絲對多醣之轉化率(Yp/x)的值作比較，結果以添加檸檬酸的轉化率最高，其值為0.26，因此以檸檬酸探討其濃度梯度對裂褶菌菌絲產量以及多醣轉化率的關係，結果添加檸檬酸的實驗組其多醣轉化率(Yp/x)約為控制組的2.2倍；而以多醣總產量而言，以添加檸檬酸比例為0.1∼0.3％的產量最高 ，但是若添加檸檬酸濃度大於0.5%則會因為過分抑制菌絲生長而導致多醣產量減少，因此在搖瓶實驗裡添加適量的檸檬酸有促進多醣產量生成的結果。第二部份為發酵槽的實驗結果，目的探討檸檬酸對裂褶菌發酵系統的動力學參數值的關係，其結果發現隨著檸檬酸濃度升高，則動力學參數m值呈現上升的趨勢，因此從實驗的結果可以推論得到檸檬酸在裂褶菌發酵系統中是扮演促進多醣的生成愈趨近為ㄧ次代謝產物的角色。

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