近幾年來，在生醫顯微顯影技術上已經有許多突破繞射極限提高螢光顯微解析度的方法問世，而其中STED（Stimulated Emission Depletion）顯微技術備受注目。STED的技術使用是兩道雷射光束來進行，一道光激發螢光基團使其發光，另一道光則使用中心光強度為零的環狀雷射光抑制所激發區域外圍的螢光，使其螢光強度透過激發射損耗過程而降低，以減少螢光光點的大小，進而提高解析度。因而許多產生環狀光束方法也被研究出，本論文將針對產生環狀光束的方法來進行研究，在此模擬以數位全像術快速地重建不同波長的環狀光束，且當改變讀取光的波長時，比較所獲得的環狀光束前後的差異。另外本論文也討論擁有線偏振以及圓偏振的環狀光束，重建前後進行強聚焦時焦點處光場的分佈情形，並且與經由Hermite高斯光束所產生擁有徑向偏振與方位角偏振的環狀光束做比較。藉由數位全像術的方法，有助於得到不同波長且不同偏振的環狀光束，並可將其適用於對環狀光束有不同要求的應用中。

In recent years, many ingenious ideas by passing the optical diffraction limit have been developed to improve the resolution in biological microscopy. Especially the Stimulated Emission Depletion microscopy (STED) was much attended. The STED technology uses two laser beams, the first one is used to excite the groups of fluorescence, the other one is a beam of zero intensity in center is used to inhibit the fluorescence surrounding the excited groups of fluorescent, so that the resolution was increased by the shrinking the area of fluorescence caused by the quenching of fluorescent intensity through the depletion from stimulated emission. Thus, various methods of generating donut-shaped beams had been researched and developed. This study is aimed to simulate the digital holography to rapidly reconstruct the donut-shaped beam and to compare the results from changing the wavelength, then to discuss the producing ways of donut-shaped beams. In addition, this study also aimes at discussing the linearly and circularly polarized donut-shaped beams , as well as the optical field distribution before and after tight focus, meanwhile comparing the donut-shaped beams formed with radial and azimuthal polarization which generated by Hermite Gaussian beams. By using the digital holography technology, we are able to obtain different donut-shaped beams with different wavelengths and polarization, which can be applied in various experiential circumstance requiring donut-shaped beams.

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