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研究生： 吳靜融
Jing-Rong Wu
論文名稱： Microhyla fissipes及其近親種在東亞及東南亞的地理親緣關係
Biogeography of Microhyla fissipes and its related species in East and Southeast Asia
指導教授： 劉阜果
Fu-Guo Robert Liu
口試委員:
學位類別： 碩士
Master
系所名稱： 生醫理工學院 - 生命科學系
Department of Life Science
論文出版年： 2020
畢業學年度： 108
語文別： 中文
論文頁數： 69
中文關鍵詞： 小雨蛙 、親緣地理 、族群遺傳 、粒腺體基因序列
相關次數： 點閱：17  下載：0 
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    • 小雨蛙(Microhyla fissipes)由Boulenger(1884)所命名，其模式地點(type locality)在台灣的台南地區，常見於台灣、中國大陸和中南半島。此物種名開始時並未被廣泛接受，直到2005年Matsui等人研究證實了分布在東亞和東南亞的族群為Microhyla fissipes的有效性。由於近年Microhyla mukhlesuri的發表，其活動範圍和Microhyla fissipes重疊，並且兩物種親緣關係並未被深入研究。因此本研究使用從台灣採集69隻 Microhyla fissipes和NCBI上公開的核甘酸序列資料進行分析，利用12S、16S和Cytb核苷酸序列進行p-distance、Bayesian演算、Maximum Likelihood和分子時鐘分析。根據演化樹和p-distance結果，東亞及東南亞小雨蛙可分為四個群：台灣中國群、中越東泰群、南越群以及西泰群，並根據模式地點推論，台灣中國群為Microhyla fissipes，西泰群為Microhyla mukhlesuri，中越東泰群和南越群可能為隱蔽物種。此外，四個群共同祖先大約開始於24,600年前開始分化，此時期正好是最近一次的大冰河時期。

    Microhyla fissipes is a species which can be easily found in Taiwan, China and Indo-China. Boulenger in 1884 reported Microhyla fissipes as a new species with type locality in Tainan, Taiwan, but not well be recognized until 2005. Besides,there was a new species named Microhyla mukhlesuri published in 2014 and its distribution was considered greatly overlapping with Microhyla fissipes’s. In addition, the phylogentic relationship of Microhyla fissipes and Microhyla mukhlesuri in East and Southeast Asia is imperative to be clarified. In this study, we used the DNA sequences of 12S,16S and Cytb acquired from 69 samples newly collected in Taiwan with published data on NCBI to investigate the phylogeoegaphy of Microhyla fissipes in East and Southeast Asia by p-distance ,the Bayesian inference ,Maximum Likelihood and Molecular clock estimates. Based on our results, we summarized that there were four groups in East and Southeast Asia, including Taiwan-China group, Mid-Vietnam-East-Thailand group, South Vietnam group,and West Thailand group. According to the species type locality, Taiwan- China group is Microhyla fissipes and West-Thailand group is Microhyla mukhlesuri. In addition, the group in South Vietnam and the group of Mid-Vietnamm-East-Thailand might be two cryptic species in Indo-China. The ancestors of these four groups started to diverse about 24,600 years ago during the latest glacial epoch period.

    中文摘要............................................................i 英文摘要...........................................................ii 誌謝..............................................................iii 目錄...............................................................iv 圖目錄..............................................................v 表目錄.............................................................vi 縮寫表............................................................vii 附錄.............................................................viii 一、緒論............................................................1 二、材料與方法....................................................7-9 2-1樣本........................................................7 2-2萃取DNA、聚合酶連鎖反應及定序...............................7 2-3粒線體基因資料蒐集與整合..................................7-8 2-4演化模式分析................................................8 2-5親緣演化分析..............................................8-9 2-6分子時鐘....................................................9 三、結果........................................................10-12 3-1序列結果...................................................10 3-2 pairwise uncorrected p-distance............................10 3-3親緣演化關係............................................11-12 3-4分子時鐘分析族群/物種分歧時間..............................12 四、討論........................................................13-16 4-1Microhyla fissipes族群.....................................13 4-2Microhyla mukhlesuri族群................................13-14 4-3隱蔽種(cryptic species)....................................14 4-4台灣的小雨蛙...............................................15 4-5建議NCBI上Microhyla ornata的物種鑑定勘誤.................16 4-6親緣演化史.................................................16 五、結論...........................................................17 六、參考文獻.......................................................18

    Atsushi T, Masafumi M, Naoko S, Wichase K, Chi‐Shiun W ,Mamoru T, Koshiro E, Kanto N, Hidetoshi O (2019) "Relict distribution of Microhyla (Amphibia: Microhylidae) in the Ryukyu Archipelago: High diversity in East Asia maintained by insularization " Zoologica Scripta , 48, 440-453
    Andreu A L, Checcarelli N, Iwata S, Shanske S, DiMauro S (2000) "A missense mutation in the mitochondrial cytochrome b gene in a revisited case with histiocytoid cardiomyopathy." Nature pediatric research , 48, 311-314
    Czernilofsky A P, Kurland C G, Stöffler G (1975) "30S Ribosomal proteins associated with the 3′-terminus of 16S RNA" FEBS Letters, 58(1), 281-284
    Castresana J (2001) "Cytochrome b Phylogeny and the Taxonomy of Great Apes and Mammals" Molecular Biology and Evolution , 18(4), 465–471
    Drummond A J, Bouckaert R R (2015) "Bayesian evolutionary analysis with BEAST" Cambridge University Press
    Eperon IC, Anderson S, Nierlich DP (1980) "Distinctive sequence of human mitochondrial ribosomal RNA genes" Nature, 286(5772), 460–467
    Hanna MG, Nelson IP, Rahman S, Lane RJ, Land J, Heales S, Cooper MJ, Schapira AH, Morgan-Hughes JA, Wood NW (1998) "Cytochrome c oxidase deficiency associated with the first stop-codon point mutation in human mtDNA" American Journal of Human Genetics, 63(1), 29–36
    Hasan M K, Islam M M, Kuramoto M, Kurabayashi A, Sumida M (2014) “Description of two new species ofMicrohyla(Anura: Microhylidae) from Bangladesh ”Zoo taxa , 3755, 401-408
    Kizaki K, Oshiro I (1980) "The origin of the Ryukyu Islands. In: Kizaki K (ed) "Natural History of the Ryukyus, Tsukiji-shokan: Tokyo ,pp. 8-37
    Matsui M, Shimada T, Ota H, Saidapur S K, Khonsue W, Tanaka-Ueno T, Wu G F (2005) "Taxonomic relationships within the pan-Oriental narrow-mouth toad Microhyla ornata as revealed by mtDNA analysis (Amphibia Anura, Microhylidae) " Zoological Science , 22, 489–495
    Matsui, M, Hamidy H, Belabut, D M, Ahmad N, Panha S, Sudin A, Khonsue W, Yong H, Jiang J, Nishikawa K, (2011) "Systematic relationships of Oriental tiny frogs of the family Microhylidae (Amphibia, Anura) as revealed by mtDNA genealogy."Molecular Phylogenetics and Evolution, 61, 167–176
    Maximov V, Martynenko A, Hunsmann G, Tarantul V (2002) "Mitochondrial 16S rRNA gene encodes a functional peptide, a potential drug for Alzheimer's disease and target for cancer therapy."Medical Hypotheses,59 (6),670–3
    Parker H W (1928) "The brevicipitid frogs of the genus Microhyla.”Annals and Magazine of Natural History,10(2),473–499
    Peter S, Ricardo D. M, João R S, Tanja D, Calvin S,Gerald S (1999) "Ubiquitin tag for sperm mitochondria. " Nature , 402, 371–372
    RamonCastro JA, Picornell A, Ramon M (1998)"Mitochondrial DNA: a tool for populational genetics studies." International Microbiology,1(4),327–332
    Weisburg W G, Barns S M, Pelletier D A, Lane D J (1990)"16S Ribosomal DNA Amplification for Phylogenetic Study " Journal of Bacteriology, 173,697-703
    Woese C.R, Fox G.E (1977)"Phylogenetic structure of the prokaryotic domain: The primary kingdoms. "Proceedings of the National Academy of Sciences the National Academy of Sciences , 74(11), 5088–5090
    Scott Freeman, Jon C Herron (2004) " Evolutionary Analysis 3rd edition " Pearson Education
    Saiki K, Nakamura H, Mogi T, Anraku Y (1996) " Probing a role of subunit IV of the Escherichia coli bo-type ubiquinol oxidase by deletion and cross-linking analyses" Journal of Biological Chemistry ,271,15336-40
    Torroni A, Achilli A, Macaulay V, Richards M, Bandelt HJ (2006) "Harvesting the fruit of the human mtDNA tree." Trends Genet ,22 (6), 339–345
    Teng LS (1990) " Geotectonic evolution of Late Cenozoic arc-continent collision in Taiwan" Tectonophysics, 183, 57-76
    Tengku Gilang Pradana, Amir Hamidy, Achmad Farajallah, Eric N. Smith (2017) "Identifikasi Molekuler Microhyla, Tschudi 1839 dari Sumatera Berdasarkan Gen 16S rRNA" ZOO INDONESIA, 26(2)
    Tajima H, Niikura T, Hashimoto Y, Ito Y, Kita Y, Terashita K, et al(2002) "Evidence for in vivo production of Humanin peptide, a neuroprotective factor against Alzheimer's disease-related insults" Neuroscience Letters, 324 (3), 227–231
    Voris, H K (2000) "Maps of Pleistocene Sea Levels in South East Asia: Shorelines, River Systems, Time Durations."Journal of Biogeography, 27, 1153-1167
    Zhi-Yong Y, Chatmongkon S, Fang Y, Nikolay A P, Sang N N, Hong-Man C, Siriwadee C, Robert W M, Jing C (2016) "Red River Barrier and Pleistocene Climatic Fluctuations Shaped the Genetic Structure of Microhyla fissipes Complex (Anura: Microhylidae) in Southern China and Indochina" Current Zoology, 62(6), 531-543
    鄭彥鵬,韓國忠,王勇,張政民(2003) "台灣島及其鄰域地層和構造特徵"，《海洋科學進展》第3期 272-280頁。
    鄧屬予(2002) "臺灣新生代大地構造"，黃奇瑜主編，中國地質學會、中國地球物理學會聯合出版。「臺灣大地構造」第一版 頁：49-93

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