| 研究生: |
陳冀嫺 Ji-Xian Li |
|---|---|
| 論文名稱: |
甘藷桃園二號對鹽分逆境之抗氧化物質的生化反應研究 Biochemical studies of salt stress on antioxidants in Ipomoea batatas (L.) Lam.cv. Taoyuan 2 |
| 指導教授: | 李賢淇 |
| 口試委員: | |
| 學位類別: |
碩士 Master |
| 系所名稱: |
生醫理工學院 - 生命科學系 Department of Life Science |
| 畢業學年度: | 89 |
| 語文別: | 中文 |
| 論文頁數: | 77 |
| 相關次數: | 點閱:9 下載:0 |
| 分享至: |
| 查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報 |
當植物遭遇環境逆境時,它會促進活性氧的生成,處於逆境下的植物
能調適或增強其抗氧化防禦系統(包括抗氧化酵素及非酵素性抗氧
化物) 。本試驗主要是研究「桃園二號葉用甘藷」於鹽分逆境下,葉
片的抗氧化酵素及非酵素性抗氧化物之變化,並且研究此甘藷在鹽分
逆境下,其抗氧化解毒機制。結果顯示桃園二號葉用甘藷之抗氧化酵
素及非酵素性抗氧化物皆會受鹽分逆境影響,在鹽分(90~150mM 氯
化鈉) 處理後,抗氧化酵素例如superoxide dismutase, catalase,
ascorbate peroxidase, guaiacol peroxidase 和glutathione reductase 之活
性有增加的趨勢,非酵素性抗氧化物例如total ascorbate 和glutathione
之含量則有減少的趨勢,葉綠素相對量則減低,離子滲漏增加。低濃
度鹽分(30~90mM 氯化鈉) 處理時,脂質過氧化會有減少的趨勢,
但高濃度鹽分(120~150mM 氯化鈉) 處理時,卻會有增加的趨勢。本
試驗結果暗示甘藷在鹽分環壓之下有調適此逆境的能力,它可能牽涉
一些關鍵的酵素性和非酵素性抗氧化系統。
generation of reactive oxygen species. Plants under stresses could modify
or increase antioxidative defense systems (include antioxidative enzymes
and non-enzymatic antioxidants). Experiments were conducted mainly to
study the changes of antioxidative enzymes and non-enzymatic
antioxidants in sweet potato (Ipomoea batatas (L.) Lam.cv. Taoyuan 2)
leaves under salt stress. An antioxidative detoxification mechanism in the
sweet potato under salt stress is also investigated. Results showed that
antioxidative enzymes and non-enzymatic antioxidants in Ipomoea
batatas (L.) Lam.cv. Taoyuan 2 were affected by salt stress. The activities
of antioxidative enzymes such as superoxide dismutase, catalase,
ascorbate peroxidase, guaiacol peroxidase and glutathione reductase
tended to increase after salt (90~150mM sodium chloride) treatments.
The contents of non-enzymatic antioxidants such as total ascorbate and
glutathione tended to decrease. The chlorophyll relative contents also
decreased. Electrolyte leakage increased. Lipid peroxidation tended to
decrease with lower concentrations of salt (30~90mM sodium chloride)
treatments, but tended to increase with higher concentrations of salt
(120~150mM sodium chloride) treatments. Results suggest that sweet
potato has an ability to respond to salt stress, they may relate to involve
of several key enzymatic and non-enzymatic antioxidant systems.
47
高景輝、林安秋. (1985). 鹽性與植物生長. 科學農業. 33: 365-368.
王嘉宏、劉麗飛、詹明才. (2000). 基因改造作物(GM 作物) 的衝擊
與未來. 科學農業. 48: 229-235.
辛仲文、姜金龍. (1999). 葉菜甘藷新品種—桃園2 號. 臺灣農業. 35:
黃士懿. (1996). 天然抗氧化劑面面觀. 中化藥訊. 30: 27-32.
馮文虎. (1994). 淺談自由基與抗氧化物. 中華民國醫檢會報. 9: 34-
歐湧淳. (1994). 自由基、活性氧與疾病. 中華民國醫檢會報. 9: 44-49.
Allen, R. D. (1995) Dissection of oxidative stress tolerance using
transgenic plants. Plant Physiol. 107: 1049-1054.
Allen, R. D., Webb, R. P. and Schake, S. A. (1997) Use of transgenic
plants to study antioxidant defenses. Free Radic. Biol. Med. 23: 473-
Alscher, R. G., Donahue, J. L. and Cramer, C. L. (1997) Reactive
48
oxygen species and antioxidants: relationships in green cells. Physiol.
Plant. 100: 224-233.
Anderson, J. V., Chevone, B. I. and Hess, J. L. (1992) Seasonal
variation in the antioxidant system of eastern white pine needles.
Plant Physiol. 98: 501-508.
Asada, K. (1992) Ascorbate peroxidase – a hydrogen peroxide –
scavenging enzyme in plants. Physiol. Plant. 85: 235-241.
Asada, K. (1984) Chloroplast: formation of active oxygen and its
scavenging. Methods Enzymol. 105: 422-429.
Beckmam, K. B. and Ames, B. N. (1998) The free radical theory of
aging matures. Physiol. Rev. 78: 547-581.
Blumwald, E., Aharon, G. S. and Apse, M. P. (2000) Sodium transport
in plant cells. Biochimica et Biophysica Acta. 1465: 140-151.
Bowler, C., Van Montagu, M. and Inzé, D. (1992) Superoxide
dismutase and stress tolerance. Annu. Rev. Plant Physiol. Plant Mol.
Biol. 43: 83-116.
Bowler, C., Van Camp, W., Van Montagu, M. and Inzé, D. (1994)
Superoxide dismutase in plants. Crit Rev Plant Sci. 13: 199-218.
49
Buchanan-Wollaston, V. (1997) The molecular biology of leaf
senescence. J. Exp. Bot. 48: 181-199.
Bueno, P., Piqueras, A., Kurepa, J., Savouré, A., Verbruggen, N., Van
Montagu, M. and Inzé, D. (1998) Expression of antioxidant
enzymes in response to abscisic acid and high osmoticum in tobacco
BY-2 cell cultures. Plant Sci. 138: 27-34.
Cakmak, I. and Marschner, H. (1992) Magnesium deficiency and high
light intensity enhance activities of superoxide dismutase, ascorbate
peroxidase, and glutathione reductase in bean leaves. Plant Physiol.
98: 1222-1227.
Cakmak, I., Strbac, D. and Marschner, H. (1993) Activities of
hydrogen peroxide-scavenging enzymes in germinating wheat seeds.
J. Exp. Bot. 44: 127-132.
Chaoui, A., Mazhoudi, S., Ghorbal, M. H. and Ferjani, E. E. (1997)
Cadmium and zinc induction of lipid peroxidation and effects on
antioxidant enzyme activities in bean (Phaseolus vulgaris L.). Plant
Sci. 127: 139-147.
Chow, C. K. (1992) Nutrition and cellular antioxidant defense. J. Chin.
Nutr. Soc. 17: 1-14.
Comba, M. E., Benavides, M. P. and Tomaro, M. L. (1998) Effect of
50
salt stress on antioxidant defence system in soybean root nodules.
Aust. J. Plant Physiol. 25: 665-671.
Cook, N. C. and Samman, S. (1996) Flavonoids – Chemistry,
metabolism, cardioprotective effects, and dietary sources. Nutritional
Biochemistry 7: 66-76.
del Río, L. A., Sandalio, L. M., Palma, J. M., Bueno, P. and Corpas, F.
J. (1992) Metabolism of oxygen radicals in peroxisomes and cellular
implications. Free Radic. Biol. Med. 13: 557-580.
Dhindsa, R. S. and Matowe, W. (1981) Drought tolerance in two mosses:
correlated with enzymatic defence against lipid peroxidation. J. Exp.
Bot. 32: 79-91.
Dhindsa, R. S., Plumb-Dhindsa, P. and Thorpe, T. A. (1981) Leaf
senescence: correlated with increased levels of membrane
permeability and lipid peroxidation, and decreased levels of
superoxide dismutase and catalase. J. Exp. Bot. 32: 93-101.
Dionisio-Sese, M. L. and Tobita, S. (1998) Antioxidant responses of rice
seedlings to salinity stress. Plant Sci. 135: 1-9.
El-Enany, A. E. (1997) Glutathione metabolism in soybean calluscultures
as affected by salinity. Biol. Plant. 39: 35-39.
51
Elstner, E. F. (1982) Oxygen activation and oxygen toxicity. Annu. Rev.
Plant Physiol. 33: 73-96.
Fadzilla, N. M., Finch, R. P. and Burdon, H. (1997) Salinity, oxidative
stress and antioxidant responses in shoot cultures of rice. J. Exp. Bot.
307: 325-331.
Foster, J. G. and Hess, J. L. (1982) Oxygen effects on maize leaf
superoxide dismutase and glutathione reductase. Phytochemistry 21:
1527-1532.
Foster, J. G. and Hess, J. L. (1980) Responses of superoxide dismutase
and glutathione reductase activities in cotton leaf tissue exposed to
an atmosphere enriched in oxygen. Plant Physiol. 66: 482-487.
Foyer, C. H. and Halliwell, B. (1976) The presence of glutathione and
glutathione reductase in chloroplasts: a proposed role in ascorbic
acid metabolism. Planta 133: 21-25.
Foyer, C. H., Descourvieres, P. and Kunert, K. J. (1994) Protection
against oxygen radicals: an important defence mechanism studied in
transgenic plants. Plant Cell Environ. 17: 507-523.
Foyer, C. H., Souriau, N., Perret, S., Lelandais, M., Kunert, K. J.,
Pruvost, C. and Jouanin, L. (1995) Overexpression of glutathione
reductase but not glutathione synthetase leads to increases in
52
antioxidant capacity and resistance to photoinhibition in poplar trees.
Plant Physiol. 109: 1047-1057.
Fridovich, I. (1986) Biological effects of the superoxide radical. Arch.
Biochem. Biophys. 247: 1-11.
Fridovich, I. (1995) Superoxide radical and superoxide dismutases. Annu.
Rev. Biochem. 64: 97-112.
Fryer, M. J. (1992) The antioxidant effects of thylakoid vitamin E (a-
tocopherol). Plant. Cell. Environ. 15: 381-392.
Gallego, S. M., Benavídes, M. P. and Tomaro, M. L. (1996) Effect of
heavy metal ion excess on sunflower leaves: evidence for
involvement of oxidative stress. Plant Sci. 121: 151-159.
Gamble, P. E. and Burke, J. J. (1984) Effect of water stress on the
chloroplast antioxidant system: alterations in glutathione reductase
activity. Plant Physiol. 76: 615-621.
Gossett, D. R., Millhollon, E. P. and Lucas, M. C. (1994) Antioxidant
response to salt stress in salt-tolerant and salt-sensitive cultivars of
cotton. Crop Sci. 34: 706-714.
Gueta-Dahan, Y., Yaniv, Z., Zilinskas, B. A. and Ben-Hayyim, G.
(1997) Salt and oxidative stress: similar and specific responses and
53
their relation to salt tolerance in Citrus. Planta 203: 460-469.
Hernández, J. A., Corpas, F. J., Gómez, M., del Río, L. A. and Sevilla,
F. (1993) Salt-induced oxidative stress mediated by activated oxygen
species in pea leaf mitochondria. Physiol. Plant. 89: 103-110.
Hernández, J. A., Olmos, E., Corpas, F. J., Sevilla, F. and del Río, L.
A. (1995) Salt-induced oxidative stress in chloroplasts of pea plants.
Plant Sci. 105: 151-167.
Hoagland, P. R. and Arnon, D. I. (1950) The water culture method for
growing plants without soil. Calif. Agric. Exp. Stn. Circ. 347.
Hodges, D. M., Andrews, C. J., Johnson, D. A. and Hamilton, R. I.
(1996) Antioxidant compound responses to chilling stress in
differentially sensitive inbred maize lines. Physiol. Plant. 98: 685-
692.
Hossain, M. A., Nakano, Y. and Asada, K. (1984)
Monodehydroascorbate reductase in spinach chloroplasts and its
participation in regeneration of ascorbate for scavenging hydrogen
peroxide. Plant Cell Physiol. 25: 385-395.
Huang, C. Y. (1996) Salt-stress induces lipid degradation and lipid phase
transition in plasma membrane of soybean plants. Taiwania 41: 96-
104.
54
Huang, C. Y. and Chen, Y. M. (1995) Role of glutathione reductase and
related enzymes in salt-tolerant mechanism of soybean plants grown
under salt-stress condition. Taiwania 40: 21-34.
Ishikawa, T., Yoshimura, K., Sakai, K., Tamoi, M., Takeda, T. and
Shigeoka, S. (1998) Molecular characterization and physiological
role of a glyoxysome-bound ascorbate peroxidase from spinach.
Plant Cell Physiol. 39: 23-34.
Iturbe-Ormaetxe, I., Escuredo, P. R., Arrese-Igor, C. and Becana, M.
(1998) Oxidative damage in pea plants exposed to water deficit or
paraquat. Plant Physiol. 116: 173-181.
Jablonski, P. P. and Anderson, J. W. (1981) Light dependent reduction
of dehydroascorbate by ruptured pea chloroplasts. Plant Physiol. 67:
1239-1244.
Jahnke, L. S., Hull, M. R. and Long, S. P. (1991) Chilling stress and
oxygen metabolizing enzymes in Zea mays and Zea diploperennis.
Plant Cell Environ. 14: 97-104.
Jiménez, A., Hernández, J. A., Barcelo, A. R., Sandalio, L. M., del
Río, L. A. and Sevilla, F. (1998a) Mitochondrial and peroxisomal
ascorbate peroxidase of pea leaves. Physiol. Plant. 104: 687-692.
55
Jiménez, A., Hernández, J. A., Pastori, G., del Río, L. A. and Sevilla,
F. (1998b) Role of the ascorbate-glutathione cycle of mitochondria
and peroxisomes in the senescence of pea leaves. Plant Physiol. 118:
1327-1335.
Kalir, A., Omri, G. and Poljakoff-Mayber, A. (1984) Peroxidase and
catalase activity in leaves of Halimione portulacoides exposed to
salinity. Physiol. Plant. 62: 238-244.
Kanofsky, J. R. and Sima, P. D. (1995) Singlet oxygen generation from
the reaction of ozone with plant leaves. J. Biol. Chem. 270: 7850-
7852.
Kato, M. and Shimizu, S. (1987) Chlorophyll metabolism in higher
plants. VII. Chlorophyll degradation in senescing tobacco leaves;
phenolic-dependent peroxidase degradation. Can. J. Bot. 65: 729-
735.
Lacan, D. and Baccou, J. C. (1998) High levels of antioxidant enzymes
correlate with delayed senescence in nonnetted muskmelon fruits.
Planta 204: 377-382.
Law, M. Y., Charles, S. A. and Halliwell, B. (1983) Glutathione and
ascorbic acid in spinach(Spinacia oleracea) chloroplasts – the effect
of hydrogen peroxide and of paraquat. Biochem. J. 210: 899-903.
56
Leshem, Y. Y. (1988) Plant senescence processes and free radicals. Free
Radic. Biol. Med. 5: 39-49.
Lidon, F. C. and Teixeira, M. G. (2000) Oxy radicals production and
control in the chloroplast of Mn-treated rice. Plant Sci. 152: 7-15.
Loggini, B., Scartazza, A., Brugnoli, E. and Navari-Izzo, F. (1999)
Antioxidative defense system, pigment composition, and
photosynthetic efficiency in two wheat cultivars subjected to drought.
Plant Physiol. 119: 1091-1099.
Mano, J., Ushimaru, T. and Asada, K. (1997) Ascorbate in thylakoid
lumen as an endogenous electron donor to photosystem II: protection
of thylakoids from photoinhibition and regeneration of ascorbate in
stroma by dehydroascorbate reductase. Photosynth. Res. 53: 197-
204.
May, M. J. and Leaver, C. J. (1993) Oxidative stimulation of
glutathione synthesis in Arabidopsis thaliana suspension cultures.
Plant Physiol. 103: 621-627.
McKersie, B. D., Bowley, S. R., Harjanto, E. and Leprince, O. (1996)
Water-deficit tolerance and field performance of transgenic alfalfa
overexpressing superoxide dismutase. Plant Physiol. 111: 1177-
1181.
57
McKersie, B. D., Chen, Y., de Beus, M., Bowley, S. R., Bowler, C.,
Inzé, D., D’Halluin, K., Botterman, J. (1993) Superoxide
dismutase enhances tolerance of freezing stress in transgenic alfalfa
(Medicago sativa L.). Plant Physiol. 103: 1155-1163.
Meneguzzo, S., Sgherri, C. L. M., Navari-Izzo, F. and Izzo, R. (1998)
Stromal and thylakoid-bound ascorbate peroxidases in NaCl-treated
wheat. Physiol. Plant. 104: 735-740.
Miyake, C. and Asada, K. (1992) Thylakoid-bound ascorbate
peroxidase in spinach chloroplasts and photoreduction of its primary
oxidation product monodehydroascorbate radicals in thylakoids.
Plant Cell Physiol. 33: 541-553.
Monk, L. S., Fagerstedt, K. V. and Crawford, R. M. M. (1989)
Oxygen toxicity and superoxide dismutase as an antioxidant in
physiological stress. Physiol. Plant. 76: 456-459.
Neumann, P. (1997) Salinity resistance and plant growth revisited. Plant
Cell Environ. 20: 1193-1198.
Osmond, C. B. and Grace, S. C. (1995) Perspectives on photoinhibition
and photorespiration in the field: quintessential inefficiencies of the
light and dark reactions of photosynthesis. J. Exp. Bot. 46: 1351-
1362.
58
Paoletti, F., Aldinucci, D., Mocali, A. and Caparrini, A. (1986) A
sensitive spectrophotometric method for the determination of
superoxide dismutase activity in tissue extracts. Anal. Biochem. 154:
536-541.
Pastori, G. M. and Trippi, V. S. (1993) Antioxidative protection in a
drought-resistant maize strain during leaf senescence. Physiol. Plant.
87: 227-231.
Pastori, G. M. and Trippi, V. S. (1992) Oxidative stress induces high
rate of glutathione reductase synthesis in a drought-resistant maize
strain. Plant Cell Physiol. 33: 957-961.
Ranieri, A., Castagna, A., Lorenzini, G. and Soldatini, G. F. (1997)
Changes in thylakoid protein patterns and antioxidant levels in two
wheat cultivars with different sensitivity to sulfur dioxide. Environ.
Exp. Bot. 37: 125-135.
Rao, M. V. and Dubey, P. S. (1993) Response of hydrogen peroxide
scavenging system in two soybean cultivars exposed to SO2:
experimental evidence for the detoxification of SO2 by enhanced
H2O2 scavenging components. Environ. Pollut. 82: 99-105.
Rodríguez-Rosales, M. P., Kerkeb, L., Bueno, P. and Donaire, J. P.
(1999) Changes induced by NaCl in lipid content and composition,
lipoxygenase, plasma membrane H+-ATPase and antioxidant enzyme
59
activities of tomato (Lycopersicon esculentum. Mill) calli. Plant Sci.
143: 143-150.
Rubinstein, B. and Luster, D. G. (1993) Plasma membrane redox
activity: Components and role in plant processes. Annu. Rev. Plant
Physiol. Plant Mol. Biol. 44: 131-155.
Salin, M. L. (1987) Toxic oxygen species and protective systems of the
chloroplast. Physiol. Plant. 72: 681-689.
Scandalios, J. G. (1993) Oxygen stress and superoxide dismutases. Plant
Physiol. 101: 7-12.
Schmidt, A. and Kunert, K. J. (1986) Lipid peroxidation in higher
plants: the role of glutathione reductase. Plant Physiol. 82: 700-702.
Seeman, J. R. and Critchley, C. (1985) Effects of salt stress on the
growth, ion content, stomatal behaviour and photosynthetic capacity
of a salt-sensitive species, Phaseolus vulgaris L. Planta 164: 151-
162.
Senaratna, T., McKersie, B. D. and Stinson, R. H. (1985) Stimulation
of dehydration injury to membranes from soybean axes by free
radicals. Plant Physiol. 77: 472-474.
Sgherri, C. L. M. and Navari-Izzo, F. (1995) Sunflower seedlings
60
subjected to increasing water deficit stress: oxidative stress and
defence mechanisms. Physiol. Plant. 93: 25-30.
Sgherri, C. L. M., Pinzino, C. and Navari-Izzo, F. (1996) Sunflower
seedlings subjected to increasing stress by water deficit: changes in
O2
·- production related to the composition of thylakoid membranes,
Physiol. Plant . 96: 446-52.
Siefermann-Harms, D. (1987) The light-harvesting and protective
functions of carotenoids in photosynthetic membranes. Physiol.
Plant. 69: 561-568.
Smirnoff, N. (1996) The function and metabolism of ascorbic acid in
plants. Ann. Bot. 78: 661-669.
Smirnoff, N. (1993) The role of active oxygen in the response of plants
to water deficit and dessication. New Phytol. 125: 27-58.
Smirnoff, N. and Colombé, S. V. (1988) Drought influences the activity
of enzymes of the chloroplast hydrogen peroxide scavenging system.
J. Exp. Bot. 39: 1097-1108.
Takahama, U. and Nishimura, M. (1975) Formation of singlet
molecular oxygen in illuminated chloroplasts. Effects on
photoinactivation and lipid peroxidation. Plant Cell Physiol. 16:
737-748.
61
Takeda, T., Yokota, A. and Shigeoka, S. (1995) Resistance of
photosynthesis to hydrogen peroxide in algae. Plant Cell Physiol. 36:
1089-1095.
Tanaka, Y., Hibino, T., Hayashi, Y., Tanaka, A., Kishitani, S., Takabe,
T., Yokota, S. and Takabe, T. (1999) Salt tolerance of transgenic
rice overexpressing yeast mitochondrial Mn-SOD in chloroplasts.
Plant Sci. 148: 131-138.
Teisseire, H. and Guy, V. (2000) Copper-induced changes in antioxidant
enzymes activities in fronds of duckweed (Lemna minor). Plant Sci.
153: 65-72.
Thompson, J. E., Ledge, R. L. and Barber, R. F. (1987) The role of
free radicals in senescence and wounding. New Phytol. 105: 317-
344.
Trippi, V. S. and Thimann, K. V. (1983) The exudation of solutes
during senescence of oat leaves. Physiol. Plant. 58: 21-28.
Tsuchihashi, H., Kigoshi, M., Iwatsuki, M. and Niki, E. (1995) Action
of b-carotene as an antioxidant against lipid peroxidation. Arch.
Biochem. Biophys. 323: 137-147.
Van Camp, W., Capiau, K., Van Montagu, M., Inzé, D. and Slooten, L.
62
(1996) Enhancement of oxidative stress tolerance in transgenic
tobacco plants overproducing Fe-superoxide dismutase in
chloroplasts. Plant Physiol. 112: 1703-1714.
Van Camp, W., Inzé, D. and Van Montagu, M. (1997) The regulation
and function of tobacco superoxide dismutases. Free Radic. Biol.
Med. 23: 515-520.
Walker, M. A. and McKersie, B. D. (1993) Role of the ascorbateglutathione
antioxidant system in chilling resistance of tomato. J.
Plant Physiol. 141: 234-239.
Wise, R. R. and Naylor, A. W. (1987) Chilling-enhanced photooxidation.
Evidence for the role of singlet oxygen and superoxide in the
breakdown of pigments and endogenous antioxidants. Plant Physiol.
83: 278-282.
Yruela, I., Pueyo, J. J., Alonso, P. J. and Picorel, R. (1996)
Photoinhibition of photosystem II from higher plants: effect of
copper inhibition. J. Biol. Chem. 271: 27408-27415.
Yu, Q. and Rengel, Z. (1999) Drought and salinity differentially
influence activities of superoxide dismutases in narrow-leafed lupins.
Plant Sci. 142: 1-11.
Zhang, J. and Kirkham, M. B. (1994) Drought-stress-induced changes
in activities of superoxide dismutase, catalase, and peroxidase in
wheat species. Plant Cell Physiol. 35: 785-791.
Zhang, J. and Kirkham, M. B. (1996) Enzymatic responses of the
ascorbate-glutathione cycle to drought in sorghum and sunflower
plants. Plant Sci. 113: 139-147.