固著於矽膠之枯草芽孢桿菌對 ECC 耐久性能的影響

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研究生：	呂伊凡 Irfan Prasetyo Loekito
論文名稱：	固著於矽膠之枯草芽孢桿菌對 ECC 耐久性能的影響 The influence of bacillus subtilis immobilized in silica gel on durability performance of ECC
指導教授：	黃偉慶 Huang, Wei-Hsing
口試委員:
學位類別：	碩士 Master
系所名稱：	工學院 - 土木工程學系 Department of Civil Engineering
論文出版年：	2023
畢業學年度：	111
語文別：	英文
論文頁數：	102
中文關鍵詞：	枯草芽孢桿菌、耐久性、氯化物擴散、腐蝕、ECC
外文關鍵詞：	ECC, Durability, Bacillus Subtilis, Chloride Diffusion, Corrosion
相關次數：	點閱：16 下載：0
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工程水泥基複合材料（ECC）作為高性能材料，通過改變粉煤灰含量、添加新型纖維、添加強添加劑化合物、摻入殺菌劑等多種方法來提高性能。開發ECC技術的目的不僅僅是提高機械性能，還包括確保耐久性能。用於評估耐久性能的特徵之一是使用壽命能力。為了延長ECC的使用壽命，必須提高一些性能，例如氯化物侵入和耐腐蝕性。為了實現高耐久性能，本研究正在研究將枯草芽孢桿菌摻入 ECC 中。為了使細菌在 ECC 混合物的惡劣環境中存活，在固定過程中使用了矽膠。在本研究中，細菌固定化矽膠的含量從 5%、10% 和 15% 不等。結果表明，利用固定在矽膠中的枯草芽孢桿菌提高了耐久性能，尤其是在乾燥收縮、氯化物擴散性和耐腐蝕性方面。

此外，ECC 的使用壽命可以通過使用本研究的表面氯化物濃度和表觀擴散係數來估算。使用有效時間方法確定時間相關的減少係數 m。由於計算的擴散係數隨時間的減少對使用壽命預測有很大影響，因此還必須知道擴散和時間相關的減少係數是如何獲得的。

As the high-performance material, engineered cementitious composite (ECC) has developed with many methods to improve the properties, such as changing the fly ash content, adding new type of fiber, adding strong additive compound, and incorporating with bacteria agent. The objective of developing ECC technology was not just about improving mechanical qualities, but also about ensuring the durability performances. One of the characteristics used to assess durability performance is service life ability. To extend the service life of ECC, some properties have to be improved, such as chloride ingress and corrosion resistance. In order to achieve high durability performance, the incorporation of bacillus subtilis into the ECC is being examined in this study. To keep bacteria alive in the harsh environment of the ECC mixture, silica gel is employed in the immobilization procedure. In this study, the content of bacteria immobilized silica gel varies from 5%, 10%, and 15%. The results reveal that utilizing bacillus subtilis immobilized in silica gel increases the durability performances, especially on drying shrinkage, chloride diffusivity, and corrosion resistance.
Furthermore, the service life of ECC can be estimated by using surface chloride concentration and apparent diffusion coefficient from this research. The time-dependant reduction coefficient, m, was determined using effective time methods. Since the reduction of calculated diffusion coefficient with time has great impact on service life prediction, it is imperative to also know how the diffusion and time-dependant reduction coefficient were obtained.

Abstract    i
摘要    ii
Acknowledgement    iii
Table of Contents    iv
List of Figures    v
List of Tables    vii
Chapter 1 Introduction    1
1 Research Background    1
2 Research Objectives    2
3 Research Scopes    2
4 Research Flowchart    2
Chapter 2 Literature Review    5
1    Nature of Reinforced Concrete Deterioration    5
2 Reinforced Concrete Deterioration Phases    6
3 Mechanism of Reinforcement Corrosion    6
4 Chloride Penetration Mechanism    8
4.1    Sorptivity or Surface Absorption    8
4.2    Diffusion    8
4.3    Chloride Binding    9
4.4    Wicking Action    9
4.5    Dispersion    9
4.6    Permeation    9
5    Chloride Diffusion    10
5.1    Diffusion Reduction Coefficient    12
6    Chloride Penetration Test    14
7    Service Life Estimation    16
8    Durability of Engineered Cementitious Composite    16
8.1    Transport Properties of ECC under Chloride Exposure    17
8.2    Corrosion Resistence Performance of ECC    17
9    Effect of Bacteria on Reinforced Corrosion    19
Chapter 3 Research Methodology    21
1    Research Program    21
2    Materials    24
2.1    Portland Cement    24
2.2    Fly Ash    24
2.3    Silica Sand    25
2.4    Polyvinyl Alcohol Fiber    26
2.5    Bacteria Specification    26
3    Bacterial Stock Preparation    27
3.1    Viability of Bacteria    27
3.2    Culturing of Bacteria    28
3.3    Immobilization of Bacteria    30
4    Mix Design    31
5    Chloride Penetration Test    33
5.1    Specimens Grinding    34
5.2    Determination of Chloride Content    35
5.3    Determination of Chloride Depth    37
5.4    Chloride Penetration Profile    38
5.5    Surface Chloride (Cs) and Apparent Diffusion (Dapp) Determination    38
5.6    Calculation of Diffusion Reduction Coefficient    39
5.7    Service Life Prediction    39
6    Accelerated Corrosion    39
6.1    Specimen Preparation    39
6.4    Gravimetric Weight Loss    40
6.2    Design of Targeted Corrosion    41
6.3    Test Setup for Accelerated Corrosion    42
7    Drying Shrinkage    44
8    Survival of Bacteria    46
Chapter 4 Results and Discussion    49
1    Survival of Bacteria in ECC Mixture    49
2    Drying Shrinkage    50
3    Microstructural Observation    52
4    Experimental Data of Bulk Diffusion    55
4.1    Chloride Penetration Depth    55
4.2    Chloride Profile of Experimental Data    56
4.3    Dapp and Cs Determination    59
4.4    Determination of m and D28 Values    64
5    Corrosion Resistance    66
5.1    Distribution of Rust Production-Induced Crack    67
5.2    Mass Loss and Actual Corrosion    68
5.3    Relationship between Diffusion and Corrosion    72
5.4    Relationship between icorr and iapp    72
6    Service Life Prediction    73
6.1    Estimation the Instataneous Diffusion Coefficient    73
6.2    Surface Chloride Estimation    74
6.3    Service Life Estimation    76
Chapter 5  Conclusions and Suggestions    81
1 Conclusions    81
2 Suggestions for Future Study    82
References    83


                                

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