本文論文提出手持式光學顯微鏡的鏡頭設計，共分為三個部分，分別為物鏡、稜鏡、目鏡的設計，設計完成之後在將這些設計組合在一起，達到顯微鏡的設計結果。物鏡的設計為10X放大率及NA為0.25，稜鏡的部分選用的是Pechan prism，目鏡的設計20X放大率，將物鏡、稜鏡、目鏡依循設計流程組合成200X放大率的顯微鏡，可以用來單獨使用於觀察物體。
物鏡與稜鏡在設計上是同一個優化系統上完成的，藉由稜鏡的設計成功得將物鏡系統的長度由40.25 mm縮短至22.00 mm，其長度縮減幅度至45.34%。在最後顯微鏡設計組裝完成時，其系統總長度達到66.23 mm，符合手持式系統的要求，並且確認其系統影像品質判斷標準以Strehl ratio大於0.8來作為其評價需求。

The design of portable microscope was introduced in this thesis. There are three parts in this design; an objective lens, a prism, and an eyepiece. Finally, combining objective lens, pechan prism and eyepiece to form an optical system.
The objective lens’s magnification and NA are abbreviated as 10X/0.25. The selection of Prism is Pechan prism. The eyepiece magnification is 20X. By lens and prism combining, we can design a 200X microscope system. It can be operated individually to observe the object.
The objective lens and the prism are built in the same optimization process. The length of objective system is reduced from 40.25 mm to 22.00 mm by the design of pechan prism. The length of the objective system is reduced by 45.34%. Finally, the total length of the microscope system is about 66.23 mm which achieves the purpose of being portable. In addition, Strehl ratio of an optical system is a diffraction limited system while the value is higher than 0.8.Therefore, the required microscope system’s Strehl ratio is higher than 0.8 to meet the image quality.

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