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研究生： 薛京
Ching Hsueh
論文名稱： 長DNA的Janus粒子
DNA-Grafted Janus Particles
指導教授： 阮文滔
Wen-tau Juan
林耿慧
Keng-hui Lin
口試委員:
學位類別： 碩士
Master
系所名稱： 理學院 - 物理學系
Department of Physics
畢業學年度： 96
語文別： 英文
論文頁數： 31
中文關鍵詞： 膠體粒子
外文關鍵詞： Janus particles, DNA
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    • 我們發展了一種新興的材料—具有”頭”與”尾”的膠體粒子。所謂”頭”，是由一種雙重化學性質的粒子作為基礎—稱作Janus 粒子; 而”尾”則是由lambda-DNA 粘在這種粒子的半面所構成。我們以兩種方法來做Janus 粒子：石臘乳膠固定法及渡金法。另外我們也測試了三種不同的生物化學鍵結將lambda-DNA 長在Janus 粒子上：生物素-卵白素鍵結、胺-乙醛鍵結、以及金-硫醇鍵結。經過結合及比較以上的方法，我們認為要做出這種”頭尾”結合的粒子用結合渡金的Janus 微米粒子與硫醇-DNA 是一個有效的方法。

    We developed novel colloidal particles which are composed of a head and tails. The “head” is based on Janus microspheres, amphiphlic particles with two different chemically modified hemispheres. The “tails”are attached by grafting l-DNA onto one of the hemisphere. Both wax-emulsion and gold-evaporation methods are used to generate Janus microspheres. We also test three different biochemical protocols to graft DNA onto silica surface: streptavidin-biotin binding, amine-aldehyde binding and thiol-gold binding. We compared all the methods and found that combining gold-evaporated Janus microspheres with thiol-DNA is an efficient way to fabricate the new class colloidal particles with head-and-tail structures.

    1. Introduction -----------------------------------------------------------------------------------1 2. Background ------------------------------------------------------------------------------------3 2.1 Colloidal Janus particles ----------------------------------------------------------------3 2.2 DNA linker ---------------------------------------------------------------------------------5 2.3 Silanization ---------------------------------------------------------------------------------7 2.4 Gold-thiol bond ---------------------------------------------------------------------------10 3. Experiment and Results ---------------------------------------------------------------------11 3.1 Synthesize Janus particles --------------------------------------------------------------12 3.1.1 Wax/water emulsion method ----------------------------------------------------12 3.1.2 Gold evaporation method --------------------------------------------------------15 3.2 DNA grafting on silica surface ---------------------------------------------------------16 3.2.1 Biotin-strepavidin binding -------------------------------------------------------17 3.2.2 Aldehyde-amine binding ---------------------------------------------------------20 3.2.3 Thiol-gold binding -----------------------------------------------------------------21 3.3 Comparison of Protocols ----------------------------------------------------------------22 3.4 Challenges ----------------------------------------------------------------------------------24 3.5 Synthesize DNA grafted Janus particles ----------------------------------------------25 3.5.1 Gold Janus Particles with Amine-aldehyde binding -------------------------26 3.5.2 Gold Janus particles with thiol-DNA -------------------------------------------28 4. Conclusion and Outlook ----------------------------------------------------------------------29

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