在現今的食品當中，潛藏著許多對人體造成健康危害的毒素，這些毒素來源有：黴菌毒素、細菌類毒素、重金屬毒素、農藥和化學污染等等，對人體可是造成疾病的發生。其中黃麴毒素AFB1為黴菌毒素的一種，主要污染來源為豆類食品。在毒性上，相對於其它具有相似結構的黃麴毒素，被認為是毒性較強的黴菌毒素。從文獻上，也發現在真菌類當中，具有其降低AFB1毒性的解毒酵素，反應後發現在螢光下偵測其AFB1吸收值降低。因此在實驗研究中，也希望能從真菌類樟芝發酵中，找出具有可降低AFB1毒性的解毒酵素。
本研究以樟芝Antrodia camphorata BCRC 35396於氣舉式發酵槽培養下，找尋具有降低毒素毒性的解毒酵素，其反應上造成AFB1螢光降低，並將發酵液進行酵素分離純化。
解毒酵素的純化步驟是將發酵澄清液利用硫酸銨沉降、透析，再經過DEAE Sepharose陰離子交換管柱層析分離純化。最後由SDS-PAGE電泳分析，找出此解毒酵素分子量為54kDa。
在反應部份，則是將純化之酵素與AFB1反應，利用AFB1在螢光excitation 365nm偵測下，emission 420nm 會有吸收值，來作為判斷毒素反應變化情況。

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