金奈米粒子合成及表面化學改質與其應用於DNA分子雜交動力學之探討

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研究生：	許景翔 Jing-Hsiang Hsu
論文名稱：	金奈米粒子合成及表面化學改質與其應用於DNA分子雜交動力學之探討 Au Nanoparticle Synthesis and Surface Chemical Modification and Its Application for DNA Hybridization Kinetics Study
指導教授：	陳文逸 Wen-Yih Chen
口試委員:
學位類別：	碩士 Master
系所名稱：	工學院 - 化學工程與材料工程學系 Department of Chemical & Materials Engineering
畢業學年度：	91
語文別：	中文
論文頁數：	71
中文關鍵詞：	去氧核醣核酸、表面化學改質、金奈米粒子、生物晶片
外文關鍵詞：	biochip, surface chemical modification, DNA, Au nanoparticle
相關次數：	點閱：14 下載：0
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本研究是利用表面化學改質的方法，在金奈米粒子(Au nanoparticle)表面形成一自我聚集單層膜(self-assembly monolayer; SAM)，探討具有不同官能基之自我聚集單層膜改質對金奈米粒子分散或聚集的影響，並可經由UV/Vis spectrophotometer中之紅移(red shift)現象來量測金奈米粒子聚集之行為。我們也藉由恆溫滴定微卡計(Isothermal Titration Calorimetry; ITC)測量出具有不同官能基單層膜之金奈米粒子的稀釋熱(dilution heat)，來表示金奈米粒子在溶液相中之分散行為。利用表面化學改質的方法與技術，可將不須經過硫修飾的單股DNA接在金奈米粒子上來當做探針(probe)，再與另一互補單股亦接在金奈米粒子上標的(target)DNA進行雜交(hybridization)，我們並改變不同的雜交環境：鹽濃度、雜交溫度及DNA序列中有錯誤鹼基配對等變因來了解其固定化於金奈米粒子的DNA分子雜交特性。本實驗之結果將有助於生物晶片及生物奈米科技之發展。

This study utilized the surface chemical modification methods to form a self-assembly monolayer (SAM) on the surface of Au nanoparticles, and discussed the aggregation or dispersion of the different functional groups SAM modified Au nanoparticles, and via the phenomena of red-shift to determine the behavior of Au nanoparticles aggregation by UV/Vis spectrophotometer. We also determined the dilution heat of different functional group SAM modified Au nanoparticles by isothermal titration calorimetry (ITC) to explain the dispersion mechanism and the behavior of Au nanoparticles in the aqueous phase. Using surface chemical modification methods and techniques, we can immobilize the unmodified single strand DNA on the Au nanoparticles as a probe, and hybridized with the complementary single strand DNA that immobilized on Au nanoparticles as a target. This study, therein investigated the DNA hybridization properties by UV/Vis spectrophotometer. The results of this investigation, hybridization properties were carried out by changing different conditions：salt concentration, hybridization temperature and number of mismatching bases in sequences of target DNA. This kind of experiment can supply the information to develop the biochip and bionanotechnology.

中文摘要.....................................................................................................I
Abstract......................................................................................................II
目錄..........................................................................................................III
圖目錄.......................................................................................................V
表目錄...................................................................................................VIII
符號說明..................................................................................................IX
第一章   緒論..........................................................................................1
第二章   文獻回顧..................................................................................3
2.1.奈米科技在生物上的應用..........................................................3
2.2.金奈米粒子的合成法..................................................................6
2.3.金奈米粒子的光學性質..............................................................9
2.4.表面化學改質............................................................................13
2.5.DNA分子的簡介.......................................................................21
第三章   實驗藥品與儀器設備............................................................24
3.1.實驗藥品....................................................................................24
3.2.儀器設備....................................................................................26
3.3.實驗目的與實驗方法................................................................26
3.3.1.實驗目的..........................................................................26
3.3.2.實驗方法.........................................................................27
3.3.2.1.金奈米粒子的製備..............................................27
3.3.2.2.不同官能基單層膜的金奈米粒子製備..............28
3.3.2.3.金奈米粒子表面化學改質及DNA分子
固定化..................................................................30
3.3.2.4.銀奈米粒子的製備..............................................31
3.3.2.5.TiO2光觸媒奈米粒子的製備..............................32
第四章   結果與討論............................................................................33
4.1.金奈米粒子合成的結果.............................................................33
4.2.羧基(-COOH)單層膜在金奈米粒子上的分散現象................…36
4.3.氫氧基(-OH)及甲基(-CH3)單層膜在金奈米粒子上的分散現象...................................................................................………. 38
4.4.利用恆溫滴定微卡計來測量金奈米粒子分散現象的熱力學機制.................................................................................................40
4.5.金奈米粒子表面化學改質及DNA固定化..................................44
4.6.DNA分子固定化於金奈米粒子的光學性質...............................46
4.7.DNA分子固定化於金奈米粒子的雜交性質.............................49
第五章   結論........................................................................................53
第六章   參考文獻................................................................................54

                                

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