利用組織工程的方法可恢復、維持或增進人體組織或器官的功能。其基本概念就是將細胞植入生物可降解的支架，細胞可在支架中分裂、分化、生存以及發揮功能，在將支架植入生物體內以修復組織與器官功能。
本研究利用冷凍擠壓層積成型(Frozen compressed deposit manufacturing, FCDM)方式製造組織工程用支架，此法由加熱擠壓層積成形(Heated compressed deposit manufacturing, HCDM)加工方法改良而成。
本系統以電腦設計出三維立體支架外形和支架內部結構的模型，再將支架模型切成多個二維切面並規劃其加工路徑。支架材料以溶劑均勻混合成適當濃度後，由x-y-z平台依規劃路徑移動噴嘴，利用高壓氣體擠壓將材料射出於一個低溫冷卻平台上使之凝固，如此一層一層建構出每個切平面，即可堆疊出複雜形狀的多孔性支架。之後將支架置入冷凍乾燥機，把溶劑抽乾去除以固化支架。
本研究使用PLGA50/50材料，以FCDM方法製作支架，並探討影響FCDM支架製作品質的相關變因如環境溫度、乾燥時間、擠製壓力與噴頭移動速度等。利用FCDM製作的支架，不會破壞材料原始特性，且適用於多樣材料的製作。經過細胞植附與機械性質等實驗，可知FCDM支架可應用於組織工程的使用。

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