本文探討二維介電質電濕式(electrowetting on dielectric, EWOD)微流體系統的設計與製作，並利用此微流體系統來做胜肽合成(Peptide Synthesis)反應的應用。利用立體結構將表面控制電極與底層的外接電路隔開，可以有效解決介電質電濕式微流體元件在增加控制電極數目成為二維陣列時，內部控制電極無法布線的問題。使用立體結構的方法製作的二維EWOD元件，在大氣環境下的操作電壓約為30伏特。其次，利用self-assembly monolayer(SAM) FTS分子鍵結的成膜方式來取代傳統使用Teflon spin-coating 方式來製作的疏水層。以此方法製作的疏水層可以防止疏水層在物理碰觸或外加高電壓時產生疏水膜破裂的現象。因此可使微流體元件更加穩固、容易清洗、耐用、亦可重複使用。
另外，利用介電質電濕式微流體元件進行胜肽合成(Peptide synthesis)反應，分別以Wang resin 和Au@Fe奈米粒子作為固體基材，分別進行兩種胺基酸的接合反應，並研究胺基酸接合情形。藉此提出以EWOD為基礎的長鏈高分子原位合成器。

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