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研究生: 謝明祐
MING-YU HSIEH
論文名稱: 臺灣大嵙崁人工濕地中水體與底泥中微型塑膠之分布特性及環境相關性分析
Distribution Characteristics and Environmental Correlations of Microplastics in Water and Sediment of The Daxi Dakekan Constructed Wetland
指導教授: 林伯勳
Po-Hsun Lin
口試委員:
學位類別: 碩士
Master
系所名稱: 工學院 - 環境工程研究所
Graduate Institute of Environmental Engineering
論文出版年: 2025
畢業學年度: 114
語文別: 中文
論文頁數: 114
中文關鍵詞: 微型塑膠人工濕地底泥環境因素去除效率
外文關鍵詞: microplastics, constructed wetland, sediment, environmental factors, removal efficiency
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  • 現今微型塑膠污染已是全球密切關注的議題之一,根據目前的研究得知各個環境或是生態幾乎都有微型塑膠的存在,生物體能透過直接攝食、吸入或皮膚接觸,以及經由食物鏈傳遞而暴露於微型塑膠,甚至人體中也有檢測出微型塑膠,因此塑膠顆粒進入到自然環境中,不僅會對生態環境造成危害,也會對人體健康產生影響。
    臺灣目前對微型塑膠的研究逐漸增多,但針對人工濕地的相關研究仍然稀少。人工濕地為一種利用自然功能進行污水處理的系統,對於去除水中微型塑膠的潛力尚未被充分探討。本研究為國內第一個針對地表流人工濕地進行微型塑膠的研究調查,自2024年8月至2025年6月於大溪大嵙崁人工濕地進行微型塑膠之採樣調查。樣品分為水樣及底泥,先經過碘化鈉密度分離後,以芬頓法去除有機物來完成前處理之步驟,尼羅紅染色法輔助螢光顯微鏡來識別微型塑膠,最後以µ-Raman光譜儀進行塑膠聚合物種類的鑑定。研究方向主要為針對大溪區及八德區的生活污水,調查其微型塑膠的豐度、形狀及聚合物類型,並且分析大嵙崁人工濕地中各池之間的豐度、聚合物、組成比例,以及觀察濕地對於微型塑膠之去除效率,再利用濕地之微型塑膠豐度和環境因子做相關性分析。
    本研究檢測出大嵙崁人工濕地有微型塑膠之存在,水樣中微型塑膠平均豐度為5.5 ± 0.9 items/L,底泥中微型塑膠之平均豐度0.9 ± 0.3 items/g dry weight。兩種樣品之整體形狀皆以碎片為主,水樣尺寸分佈皆以74 ~ 100 μm為主,底泥尺寸分佈則大部分皆為300 μm以下。環境樣品中檢測出聚丙烯(PP)、聚乙烯(PE)、聚苯乙烯(PS)、聚對苯二甲酸乙二醇酯(PET)、聚氯乙烯(PVC)、聚醯亞胺(PI)六種聚合物類型。而濕地系統對於微型塑膠之去除效率大約為52~53%。相關性分析顯示,水體中微型塑膠豐度與當日及前24小時降雨量呈顯著正相關,底泥中微型塑膠豐度與降雨量皆呈無顯著相關。


    Microplastic (MP) pollution has become one of the most pressing global environmental concerns. Current research has revealed the widespread presence of microplastics across various environmental compartments and ecosystems. Organisms can be exposed to MPs through direct ingestion, inhalation, dermal contact, or trophic transfer, and MPs have even been detected in human tissues. Consequently, the release of plastic particles into the natural environment poses potential threats to both ecosystems and human health.
    In Taiwan, studies on microplastics have gradually increased in recent years; however, research focusing on constructed wetlands remains limited. Constructed wetlands are nature-based systems designed for wastewater treatment, yet their potential to remove MPs has not been fully explored. This study represents the first investigation of MPs in a free water surface constructed wetland (FWS-CW) in Taiwan. From August 2024 to June 2025, samples were collected from the Daxi Dakekan Constructed Wetland. Both water and sediment samples were analyzed. Samples underwent density separation using sodium iodide (NaI), followed by Fenton’s reagent digestion to remove organic matter. MPs were identified using Nile Red staining under a fluorescence microscope, and polymer types were determined using µ-Raman spectroscopy.
    The research focused on MPs originating from domestic wastewater in Daxi and Bade Districts, examining their abundance, shapes, and polymer types, as well as spatial variations and removal efficiencies across different wetland units. Correlations between MP abundance and environmental parameters were also analyzed.
    Results confirmed the presence of MPs in the Dakekan Constructed Wetland, with an average abundance of 5.5 ± 0.9 items/L in water samples and 0.9 ± 0.3 items/g dry weight in sediment samples. Fragments were the dominant shape. Water samples were mainly composed of particles sized 74~100 μm, while most sediment particles were <300 μm. Six polymer types were identified: polypropylene (PP), polyethylene (PE), polystyrene (PS), polyethylene terephthalate (PET), polyvinyl chloride (PVC), and polyimide (PI). The overall MP removal efficiency of the wetland was approximately 52–53%. Correlation analysis showed a significant positive relationship between MP abundance in water and rainfall on the sampling day and within the previous 24 hours, whereas no significant correlation was observed for sediment samples.

    致謝 i 摘要 ii Abstract iii 目錄 v 圖目錄 viii 表目錄 ix 第一章 前言 1 1.1 研究緣起 1 1.2 研究目的與重要性 2 第二章 文獻回顧 4 2.1 塑膠之起源 4 2.2 微型塑膠之定義 5 2.3 微型塑膠之來源 6 2.4 微型塑膠之影響 7 2.4.1 微型塑膠對於人體之影響 7 2.4.2 微型塑膠對於環境之影響 9 2.5 人工濕地裡的微型塑膠 10 2.5.1 人工濕地對於微型塑膠之去除能力 10 2.5.2 植物對於微型塑膠之去除能力 13 2.5.3 動物對於微型塑膠之去除能力 14 2.5.4 微型塑膠與降雨之相關性 15 2.6 微型塑膠檢測方法 17 2.6.1 底泥及水樣採樣方式 17 2.6.2 微型塑膠之前處理 19 2.6.2.1 水樣之前處理 20 2.6.2.2 底泥之前處理 21 2.6.2.3 植物之前處理 22 2.6.2.4 消化法 23 2.6.3 微型塑膠分析方式 24 2.6.3.1 識別方法 24 2.6.3.2 鑑定聚合物 25 2.7 微型塑膠研究之品質控管與回收試驗 27 2.7.1 QA/QC 27 2.7.2 回收率試驗 28 第三章 研究材料與方法 29 3.1 研究架構 29 3.2 研究區域 31 3.3 採樣方法 34 3.4 微型塑膠之前處理 35 3.4.1 無機物之分離 36 3.4.2 有機物之氧化 38 3.4.3 微型塑膠之識別 40 3.4.4 微型塑膠之鑑定 41 3.5 實驗用藥品及溶液 42 3.6 品質保證與品質控制 42 3.6.1 污染控制措施 43 3.6.2 空白試驗 44 3.6.3 微型塑膠之密度分離回收試驗 45 3.7 統計分析 46 第四章 結果與討論 47 4.1 空白試驗 47 4.1.1 空氣空白 47 4.1.2 實驗程序空白 48 4.2 微型塑膠回收試驗 49 4.3 微型塑膠準確率 50 4.4 人工濕地之微型塑膠分佈 51 4.4.1 人工濕地水樣之微型塑膠豐度 52 4.4.2 人工濕地底泥之微型塑膠豐度 56 4.4.3 人工濕地微型塑膠在不同採樣時間之形狀分布 59 4.4.4 人工濕地微型塑膠在不同採樣時間之尺寸分布 62 4.4.5 人工濕地各池間微型塑膠形狀及尺寸分佈 65 4.5 人工濕地與降雨量之相關性 69 4.6 人工濕地微型塑膠之去除效率 72 4.7 人工濕地中微型塑膠之聚合物類型 75 第五章 結論與建議 79 5.1 結論 79 5.2 建議 80 參考文獻 81

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