裂褶菌為食藥用菇中的一種，食藥用菇最吸引人的地方在其抗癌、抗腫瘤的效果，而裂褶菌於液態葡萄糖培養基培養時產生的胞外多醣，稱為裂褶菌多醣，主要的成份為β-1-3/β-1-6 glucan，經學者確認此結構具有抗癌、抗腫瘤療效，而且經動物與人體試驗證實能有效地提高其免疫機能，活化體內巨噬細胞的吞噬能力，而且裂褶菌多醣可開發為醫藥治療用途，用於幫助增強病人的免疫系統和抵抗腫瘤的能力，因此具有相當潛力被開發為未來醫療用品，或作為近年來國內相當熱門的保健食品，視為未來的明星產品。
為了有效增加裂褶菌多醣的產量，並縮短生產的時間，本研究將利用液態深層發酵來培養生產裂褶菌多醣，採用的菌株為裂褶菌Schizophyllum commune ATCC 38548，其中影響發酵過程的因素很多，本研究將針對培養基的改變與氧氣的問題，探討對裂褶菌生產的影響。據文獻指出，麩胺酸能有效幫助一些微生物於生產過程中提供能量以利於菌體生長和促進多醣生成的增加，因此實驗中選擇添加麩胺酸來探討其對於裂褶菌菌體生長和多醣生成的影響，並利用動力學參數模擬計算出各種參數值，藉由參數值的關係推測麩胺酸與裂褶菌代謝機制的關連性。另外，由於好氧菌進行發酵培養時，氧氣為菌體代謝反應中的限制因子，用以產生能量提供細胞活性的作用，而且根據文獻指出，供氧量的多寡對於菌體生長和多醣生成都有舉足輕重的影響，因此本研究另一項考慮的變因就是供氧量的問題，希望藉由不同供氧量的關係，釐清氧氣在裂褶菌發酵培養中所扮演的角色。最後採用兩階段操作模式，做不同的麩胺酸濃度和供氧量的調控，尋求最適合裂褶菌生產的條件。
在搖瓶實驗中發現，添加麩胺酸有助於多醣產量的增加與轉化率的提升，在菌體濃度方面則因為添加過高濃度的麩胺酸(0.5%)，而有抑制菌體生長的現象發生，而搖瓶處於缺氧的狀態下培養，多醣產量高於發酵槽環境，顯示缺氧的狀態下有利於菌體刺激更多的多醣生成。
在發酵槽培養實驗中發現，添加麩胺酸有助於菌體比生長速率的提升，且多醣產量、產率以及轉化率都有明顯增加的趨勢，其中添加5 g/L麩胺酸所得到的產率達0.57 g/L‧day，相較於控制組(0.29 g/L‧day)增加了1.97倍，顯示添加麩胺酸會促使裂褶菌改變其產物代謝途徑，刺激多醣的生成。探討麩胺酸對裂褶菌發酵系統的動力學參數值關係中，其結果顯示隨著麩胺酸濃度升高，動力學參數m值呈現上升的趨勢，因此可推測麩胺酸在裂褶菌發酵系統中是扮演促使多醣生成趨於一次代謝產物的角色。
在改變供氧量的發酵槽實驗中發現，最大菌體濃度、比生長速率以及菌體轉化率都隨著供氧量的降低而有下降的趨勢，顯示低溶氧的狀態不利於菌體的生長；不過對於多醣的產量、產率和轉化率卻是有相當程度的幫助，其中低溶氧(0.05 vvm)的產率為0.406 g/L‧day，相較於高溶氧(0.5 vvm)的0.29 g/L‧day，提高了1.4倍。而兩階段不同供氧量的操作上，發現無論是多醣產量、產率或是轉化率都較單一固定供氧量的結果來的好，其中產率可達0.698 g/L‧day，對於高、低供氧量而言分別增加了2.41與1.72倍。
兩階段的饋料批次操作，得到的多醣濃度效果都比批次操作來的好，其中又以同時添加麩胺酸和葡萄糖將更有助於裂褶菌多醣之生成，產率可高達1.022 g/L‧day，相較於控制組(未添加麩胺酸)的批次發酵結果0.29 g/L‧day，產率提高3.52倍之多。

Cell growth and schizophyllan formation of Schizophyllum commune were greatly influenced by the glutamic acid.Effects of glutamic acid on schizophyllan batch culture were investigated in the batch cultures by glutamic acid supplemented initially from 0 to 5 g/L。The cell growth rate was increased from 0.489 to 0.672 day-1, the productivity was enhanced from 0.29 to 0.57 g/L.day。The product formation kinetics was mixed-type kinetic, and the behavior of product formation was likely to be growth-associated as the concentration of glutamic acid increased.

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