本實驗藉由幾丁聚醣與海藻膠的複合反應在兩高分子溶液以不同重量比例，pH值變化及鈣離子交聯時間等不同條件下來形成孔洞之板狀結構物以降低其剛性、增加吸收傷口滲出液及氧氣透過的能力。
此幾丁聚醣/海藻膠複合薄膜在水含量測試有不錯的吸水值。其中又以改變pH值系列可得到較高的水含量，氣體透過實驗顯示由於薄膜具高孔洞結構而能完全透過，水氣透過值則約在1300~1400(g＊m-2day-1)而不會使水分散失太快。經由3T3纖維母細胞生物體外毒性實驗(in vitro)顯示此複合薄膜不但不會釋放有毒物質，且其薄膜之立體結構可提供細胞生長的空間，有助於組織修復。
包覆AgSD之幾丁聚醣/海藻膠複合薄膜其載藥率可達到87~91%，藥物可持續釋放達14天之久。藉由改變兩高分子溶液pH值讓複合反應的鍵結增強，使得此系列的藥物釋放速率較重量比例改變系列來的緩慢。抗菌實驗顯示經包覆AgSD的複合薄膜對於綠膿桿菌、金黃葡萄球菌都有明顯的抗菌能力，且其抗菌能力能維持至七天以上。

Chitosan [β（1→4）-2-amino2-deoxy-D-glucan] is a nontoxic, biocomptible polymer and widely be used for wound dressing material. Three conditions of the experiment were employed: the different weight ratio and pH change of the polymer solution and crosslinking with calcium ion via different time. The homogeneous composite membrane consist of the porous structure which act as the water vapor barrier and is capable of absorbing wound exudate and provides a moist environment.
Due to the porous sponge structure of the composite membrane, oxygen could pass membrane completely and the membrane had better water uptaking ability, especially in pH change series. In vitro experiment, the composite membrane was nontoxic to the fibroblast cells and provided three-dimensional structure for cell growth and tissue repiration.
The release of AgSD from the composite membrane was slowly up to 14 days. Owing to the stronger bonding complex reaction the AgSD release of the pH series was slower than that the different weight ratio series. The antibacterial capacity of AgSD-impregnated wound dressing was examined in agar plate against Pseudomonas aeruginosa and Staphylococcus aureus. It was significantly found that the suppression of bacterial proliferation was effective within 7 days when compared with control group.

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