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研究生： 王介光
Chieh-Kuang Wang
論文名稱： 溫度不敏感性之電動力學行為於毛細管區域電泳
Temperature-Insensitive Electrokinetic Behavior in Capillary Zone Electrophoresis
指導教授： 曹恒光
Heng-Kwong Tsao
口試委員:
學位類別： 碩士
Master
系所名稱： 工學院 - 化學工程與材料工程學系
Department of Chemical & Materials Engineering
畢業學年度： 92
語文別： 中文
論文頁數： 60
中文關鍵詞： 電雙層 、電滲流 、電泳動 、毛細管區域電泳法 、焦耳熱效應 、非直接性測量法
外文關鍵詞： On-Column detection, electrophoretic mobility, Joule heating, Indirect UV detection, Electroosmotic mobility, capillary zone electrophoresis
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    • 在毛細管區域電泳實驗中，通常在高電壓下實驗會產生焦爾熱效應，其主要原因是因為毛細管內溫度控制及熱消散問題。
    通常我們利用電壓和(電滲流或電泳動)泳動度作圖，在低壓下是符合歐姆定律，但在高壓下焦爾效應產生，隨著毛細管內部溫度無法有效的消散，造成管內溫度上升，進而使溶液黏度下降。我們經由簡單溫度分析去了解到毛細管內部的熱傳效應，發現電滲流泳動度和電流值呈現線性比例關係，而且可以忽略毛細管內焦爾效應和不均勻的熱對流效應。將這種把溫度效應相消的結果稱作溫度的不敏感性，類似Walden’s 定律可將黏度和電流值比率得到一常數的關係。我們設計一連串的實驗，去證實溫度不敏感性和電動力學性質的行為。最後我們建議要避免焦爾效應於不均勻的溫度控制下，最好是利用電流作為控制的主要參數，而非電壓。

    Capillary zone electrophoresis generally suffers the Joule effect at relatively high driven voltage owing to the temperature control problem. In the plot of migration velocity against voltage, the electroosmotic (and electrophoretic) mobility remains a constant at lower voltage but becomes to grow at higher voltage. It is attributed to the reduction of the solvent viscosity caused by the temperature increase. A simple analytical theory is derived to show that the electroosmotic velocity is linearly proportional to the electric current regardless of the temperature profile inside the capillary due to Joule heating and non-uniform surrounding convection. This temperature-insensitive behavior is caused by the cancellation of the temperature effects such as the Walden’s rule for the constant product between solvent viscosity and electric conductivity. A series of experiments has been performed to confirm the temperature-insensitive electrokinetic behavior. Our results suggest that the Joule effect can be circumvented by controlling the electric current even under non-uniform surrounding convection.

    第一章 緒論……………………………………………………………1 第二章 毛細管電泳簡介………………………………………………3 2-1 毛細電泳儀裝置…………………………………………4 2-1-1 毛細管介紹………………………………………5 2-1-2 電源供應器……………………………………….6 2-2 毛細管電泳原理………………………………………….6 2-2-1 電泳動和電滲流…………………………………..6 2-2-2 電滲流的機制……………………………………..8 2-2-3 電滲流的在分離與分析上優點………………….11 2-2-4 電滲流的泳動度(mobility)理論與實驗估算……12 2-2-5 各種影響電滲流的因素………………………….13 2-2-6 焦耳熱……………………………………………16 2-2-7 電泳動的泳動度(mobility)理論與實驗估算……17 第三章 電滲流與微流體文獻回顧…………………………………....20 第四章 實驗裝置和方法………………………………………………25 4-1 實驗藥品…………………………………………………25 4-2 實驗儀器………………………………………………….25 4-3 實驗步驟………………………………………………….27 第五章 結果與討論……………………………………………………29 5-1 電滲流再現性檢驗……………………………………….29 5-2 非直接性測量電滲流…………………………………….30 5-3 簡單溫度分析…………………………………………….34 5-4 不同緩衝溶液濃度下的電滲流實驗…………………….37 5-5 不同緩衝溶液濃度下的電泳動實驗…………………….44 5-6 電導度計估算界劑離子泳動度………………………….48 第六章 結論和建議……………………………………………………49 第七章 參考文獻……………………………………………………50

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