本研究主要探討25 下，DMPC/PF-127（重量比為34：4、34：14和34：34）之Gibbs’吸附單分子層和PF-127分子吸附於三種不同單分子層（DMPC、DMPC/Vit-E及DMPC/cholesterol）的氣/液界面行為。首先就DMPC/PF-127 Gibbs’吸附單分子層系統而言，主要進行平衡表面張力量測和表面壓-每分子佔據面積等溫線（Π-A isotherm）的實驗。在所得的結果中，平衡表面張力隨著 PF-127添加量的增加呈現正比的線性關係。配合Gibbs表面過剩濃度關係式，可得表面過剩濃度和分子佔據面積。混合單分子層表面壓-每分子佔據面積的量測中，隨著PF-127添加量的增加，等溫線向右平移且有相變區（liquid-expanded transition liquid-condensed）的發生。
PF-127分子吸附在DMPC、DMPC/Vit-E、DMPC/ cholesterol三種不同單分子層的行為，則是藉由量測表面壓隨時間的變化的量測和表面壓-每分子佔據面積等溫線來進行研究。PF-127吸附在DMPC單分子層系統中，不同起始表面壓之表面壓隨時間變化的結果顯示，平衡表面壓與PF-127添加量呈正比關係。對於DMPC/Vit-E和DMPC/cholesterol混合單分子層系統，平衡表面壓的範圍分別是從 15.8∼28.3mN/m和 20.3~27.7mN/m，顯示添加維他命E和膽固醇改變分子間排列距離，使PF-127吸附於單分子層的表面壓產生差異。PF-127吸附平衡後之Π-A等溫線的結果顯示：（1）等溫線中有相變區的發生；（2）曲線向右平移，表示PF-127分子可能吸附或嵌入單分子層中，但因吸附的不夠緊或嵌入的不深，在高表面壓時，PF-127易被擠出單分子層中。此與微脂粒DSC實驗中，無論是嵌入法或吸附法都不影響相轉移溫度，推測PF-127嵌入脂質雙層不深是相符的。

This study investigated the behavior of mixed DMPC/PF-127 Gibbs’ adsorption monolayers (the weight ratio are 34: 4 , 34:14 and 34:34)and the PF-127 absorbed on different monolayers(the composition are DMPC, DMPC/Vit-E and DMPC/cholesterol)at the air/liquid interface at 25 . For mixed DMPC/PF-127 adsorption monolayers system at the air/water interface, the measurements of equilibrium surface tensions and surface pressure-area per molecule(Π-A) isotherms were carried out. The results of the mixed monolayer, the equilibrium surface tensions increased with the PF-127 concentration. But the excess of surface concentration is non- linear. TheΠ-A isotherms of the behavior of mixed DMPC/PF-127 monolayers indicate that the addition of the PF-127increased the shift percentages and the transition phases were obviously.
The behavior of the PF-127 adsorbed on different monolayers were investigated from the measurements of the change of surface pressure with time andΠ-A isotherms. In the PF-127 adsorption of monolayer systems, the equilibrium adsorption surface pressure increased with the PF-127 concentration and the initial surface pressure. The behavior of PF-127 adsorbed on DMPC/Vit-E and DMPC/cholesterol mixed monolayers, the ranges of equilibrium adsorption surface pressures are 15.8~28.3mN/m and 20.3~27.7mN/m, indicated that the addition of α-tocopherol and cholesterol change the distance of mixed monolayers. TheΠ-A isotherms of the PF-127 adsorbed to balance indicate that the transition phases were carried out and the PF-127 might be insert or absorb to monolayers. However, at higher surface pressure, the PF-127 molecules are squeezed into the subphase. From the DSC (differential Scanning Calorimetry ) experiments of liposome, the change of temperatures of phase transition regardless of the PF-127 incorporated or adsorbed to liposomes, which suggest the PF-127 does not insert or absorb deeply. Finally, the results obtained in this study support that the PF-127 can be readily absorbed or inserted onto liposome, providing steric barrier , preventing the fusion or aggregation of liposomes.

1. Bangham, A. D., M. M. Standish, and J. C. Watkins, “Diffusion of Univalent Ions across the Lamellae of Swollen Phosopholipids”, Journal of Molecular Biology, 13,1965, 19 238-252
2. Cevc, G., Liposome Technology：Liposome Preparation and Related Techniques, Gregoriadis , G., RD.; CRC Press : Boca Raton , Florida, 1993 , vol.Ι, Chapter1.
3.BASF Operating Manual , BASF Corporation , Germany
4.Kostarelos, K., P. F. Luckham, and T. F. Tadros, ”Addition of (Tri-)block Copolymers to Phospholipid Vesicles: A Study of Molecular Morphology and Structure by Using Hydrophobic Dye Molecules as Bilayer Probes”, Journal of Colloid and Interface Science, 191, 341-348(1997)
5.Kostarelos, K., T. F. Tadros, and P. F. Luckham, ”Physical Conjugation of (Tri-)block Copolymers to Liposomes towards the Construction of Sterically Stabilized Vesicle Systems”, Langmuir, (1999)369-376
6.Koga. T. and T. Terao, Antioxidant Behaviors of Vitamin E analogues in unilamellar vesicles, Biosci. Biochem ., 60 (1996) 1043-1045
7.Slotte,J.P., Lateral domain heterogeneity in cholesterol/ phosphatidylcholine monolayers as a function of cholesterol concentration and phosphatidylcholine acyl chain length, Biochimica et Biophysica Acta 1238(1995)118-126
8.Ghannam, M. M., M.M. Mohsen, W. A. Khalil, Interaction of type-I collage with phospholipid monolayer, Biophysical Chemistry 80 (1999) 31-40
9.Liu, D.Z.,W.Y. Chen, L.M. Tasi, S.P. Yang, Microcalorimetric and shear studies on the effects of cholesterol on the physical stability of lipid vesicle, Colloids and Surfaces A: Physicochemical and Engineering Aspects 172(2000)57-67
10.Liu, D.Z., W.Y. Chen, L.M. Tasi, S. P. Yang, The effects of cholesterol on the release of free lipids and the physical stability of lecithin liposome. J.Chin. Inst. Chem. Engrs. 31(2000) 269-273
11.Kostarelos, K., P. F. Luckham , and T. F. Tadros, Steric Stabilization of Phospholipid Vesicle by Block Copolymers: Vesicle Flocculation and Osmotic Swelling Caused by Monovalent and Divalent Cations, Journal of Chemical Society-Faraday Transactions, 94, 15, 2159-2168(1998)
12.Kirby, C., and Gregoriadis, G., Plasma-Induced Release of Solutes from Small Unilamellar Liposome Is Associated with Pore Formation in Bilayers, The Biochemical Journal 199, 1981, 251
13.Khand, L., A. Rogerson, , G.W. Halbert, A.J. Baillie, and Florence, A.T.,The Effect of Cholesterol on the Release of Doxorubicin from Nonionic Surfactant Vesicles, Journal of Pharmacy Pharmacolgy, 39,41,1987
14.Macdonald, R.C., in Vesicle, M. Rosoff, , Ed., Marcel Dekker, Inc.:New York, 1996, Chapter 1.
15.Bin, H., L.Z. Mi, S.F. Sui, Study of spontaneous insertion of non─ionic surfactant Triton X-100 into phospholipid monolayers, Thin Solid Films 327-329 (1998) 69-73
16.Ghannam, M. M., M. M. Mady, W. A. Khalil , Interaction of type-I collage with phospholipid monolayer , Biophysical Chemistry 80 (1999) 31-40
17.李松伯，界面活性劑混合增效作用對表面張力及微胞形成的影響，碩士論文，國立成功大學化學工程研究所，民國86年。
18.Shaw, D. J., 膠體及界面化學入門（introduction to Collid and Surface Chemistry 4/E）, 高立書局1997
19. Myers, D., Surfaces, Interfaces, and Colloids, VCH ,1991 , Chapter 3.
20.Birdi, K. S., Lipid and Biopolymer Monolayers at Liquid Interfaces, New York, 1989
21.Jones, M.N., and D. Chapman., Micells, Bilayers, and Biomembranes, Wiley-Liss, Inc.: New York, 1995, Chapter 4.
22.Alexandridis P., T.A. Hatton, Poly(ethylene oxide)-poly(propylen oxide)-poly(ethylene oxide) block copolymer surfactants in aqueous solutions and at interfaces : thermodynamics , structure , dynamics , and modeling , Colloids and Surfaces A: Physicochemical and Engineering Aspects 96 (1995) 1-46
23.Lisi, R. D., and S. Milioto , Poly(ethylene oxid)13-poly(propylene oxide)30-poly(ethylene oxide)13 electrolyte interactions in aqueous solutions at some temperatures , Langmuir 2000 , 16 , 5579-5583
24.Marco , V. D. W. , J. Hoechstetter , W.E. Hennink , D. J.A. Crommrlin , The effect of a water/organic solvent interface on the structural stability of lysozyme , Journal of controlled release 68 (2000) 351-359
25.Iain F. Paterson, Babur Z. Chowdhry, Stephen A. Leharne, An investigation of adsorption at the air-water and soil-water interfaces for non-micellizing ethylene oxide-propylene oxide surfactants, Chemosphere 40 (2000) 1399-1405
26.Alexandridis, P., and L. Yang, SANS Investigation of polyether block copolymer micelle structure in mixed solvents of water and formamide , Ethanol , or Glycerol , Macromolecule 2000, 33 , 5574-5587
27.Alexandridis, P., J. F. Holzwarth, and T. A. Hatton, ”Micellization of Poly(ethylene oxide)-Poly(propylene oxide)-Poly(ethylene oxide) Triblock Copolymers in Aqueous Solutions:Thermodynamics of Copolymers Association”, Macromolecules, 27,1994, 2414-2425
28.Sabagh A.A.M., N G.. Kandil , A.M. Badawi, H. El-Sharkawy, Surface activity and thermodynamic of micellization and adsorption for isooctylphenol ethoxylates , phosphate esters and their mixtures with N-diethoxylate perfluorooctanamide , Colloids and Surfaces A :Physicochemical and Engineering Aspects 170 (2000) 127-136
29.James, R. C., and R.D. Tilton, A scanning angle reflectometry investigation of block copolymer adsorption to insoluble lipid monolayers at the air-water interface, Journal of Physical Chemistry 1996, 100 , 3179-3189
30.Amélia, M.,G.D. Silva, M. I. Viseu, Synergism in mixed monolayers of cationic and anionic surfactants: A thermodynamic analysis of miscibility at the air—water interface , Colloids and Surfaces A: Physicochemical and Engineering Aspects Volume: 144, Issue: 1-3, December 20, 1998, pp. 191-200
31.Makino, K., T. Yamada, M Kimura.T Oka, H Ohshima, and T. Kondo, Temperature-Induced and Ionic Strength-Induced Conformational-Changes in the Lipid Head Group Region of Liposomes as Suggested by Zeta-Potential Data, Biophysical Chemistry, 41, 175-183(1991)
32.Lasic, D. D., Liposomes：from Physics to Applications, Elsevier Science Publishers B.V., Amsterdam, 1993
33.Schneider, P.B., H.K. Cammenga, Relation between three-dimensional phase behavior and spreading properties of DMPC, Thin Solid Films 284-285(1996) 27-31
34.周宗瀚，含DPPC之混合單分子層的分子間交互作用力，碩士論文，國立成功大學化學工程研究所，民國86年。
35.Chou, T.H., C.H.Chang, Thermodynamic behavior and relaxation processes of mixed DPPC/cholesterol monolayers at the air/water interface, Chou, Colloids and Surface B: Biointerface 17(2000) 71 - 79
36.Davides, J.T., and E.K. Rideal, Interfacial Phenimena , 2nd Edition, Academic, New York, 1963
37.Chapman, D., N.F. Owens, M.C.Phillips and D.A. Walker, Mixed Monolayers of Phosphlipid and Cholesterol, Biochemical et Biophysica Acta 183 (1969) 458-465
38.Martain, R.L., C.Washinhton, and S.S.Davis, Evidence for the Formation of Amphotericin B-Phospholipid Complex in Lanhmuir Monolayers, Pharmaceutical Research 13 (1996) 1008-1014
39.Petter, M., B. Robert, and J.P. Slotte, Molecular Interaction and Lateral Domain Formation in Monolayers Containing Cholesterol and Phosphatidylcholines with Acyl- or Alky-Linked C16 Chain, Langmuir 12 (1996) 1284-1290
40.Rosario, M.R.N., J.W. Peter, C.C. David, and J.M.R.Patino, Surface Dilational Properties of Protein and Lipid Films at the Air-Water Interface, Langmuir 14 (1998)2160-2166
41.刈米孝夫，界面活行記的原理與應用，高立圖書，1986。
42.羅浚育，界面活性劑與微脂粒的應用，碩士輪文，國立中央化工程研究所，民國89年。
43.廖明隆編譯，界面化學與界面活性劑，文源書局，民國83年再版。
44.Martin, P. and M. Szablewski, Tensiometers and Langmuir-Blodgett Troughs, Operating manual 5th Edition, Frank Grundfeld Ed, Nima, 1999
45.Chattoraj, D. K., D.K. Chattoraj and K.S. Birdi.Adsorption and The Gibbs Surface Excess, New York : Plenum Press,1984
46.Donohue, D.D., G.L.Aranovich, Classification of Gibbs adsorption isotherm, Advances in Colloid and Interaface Science76-77 (1998)137-152
47.Wu, J., J.B. Li, J. Zhao, R. Miller , Dynamic harcterization of phosopholipid/protein competitive adsorption at the aqueous solution/chloroform interface, Colloids and Surfaces A : Physicochemical and Engineering Aspects 175 (2000) 113-120
48.Junbai, L., R. Miller, R. Wustneck, H. Mohwald, A. W. Neumann , Use of pendent drop technique as a film balance at liquid/liquid interfaces , Colloids and Surfaces A :Physicochemical and Engineering Aspects 96 (1995) 295-299
49.Jubbard, J.B, V. Silin, A.L. Plant, Self assembly driven by hydrophobic interactions at alkaenthiol monolayers: mechanism of formation of hybrid bilayer membranes, Biophysical Chemistry 75 (1998) 163-176
50.Marsh, D., CRC Handbook of Lipid Bilayers, CRC Press, Boca Raton, 1990
51.楊淑萍，三團聯共聚物及鎂離子對微脂粒物理穩定性之影響及其機制，國立中央大學化工所，民國89年
52.Gershfled, N.L., and R.E.Pagano, Physical Chemistry of Lipid Flims at the Air-water Interface.Ⅲ. The Condensing Effects of Cholesterol. A Critical Examination of Mixed-Film Studies, Journal of physical and Chemica, 76(1972) 1244,
53.Shah, D. O. and J.H. Schulman , Influence of Calcium, Cholesterol, and Unsaturation on Lecithin Monolayers, J. Lipid Res., 8, 215, 1967
54.Shah, D. O. and S. Y Shiao, Monolayers Advance in Chemistry Series 144, Goddard ,E.D., Ed., American Chemical Society , Washington, DC, 153, 1978
55.Cadenhead, D. A. and F.Muller-Landau, Molecular Accommodation and Molecular Interactions in Mixed Insoluble Monomolecular Films, Journal of Colloid and Interface Science, 78, 269, 1980