探討利用PDMS sponge進行萃取式發酵以提升Clostridium acetobutylicum 產丁醇之研究

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研究生：	陳泰源 Tai-yuan Chen
論文名稱：	探討利用PDMS sponge進行萃取式發酵以提升Clostridium acetobutylicum 產丁醇之研究
指導教授：	徐敬衡
口試委員:
學位類別：	碩士 Master
系所名稱：	工學院 - 化學工程與材料工程學系 Department of Chemical & Materials Engineering
論文出版年：	2015
畢業學年度：	103
語文別：	中文
論文頁數：	67
中文關鍵詞：	疏水性海綿、丁醇
外文關鍵詞：	PDMS sponge
相關次數：	點閱：11 下載：0
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本研究為利用疏水性海綿對Clostridium acetobutylicum進行萃取式發酵，並探討其影響及可行性。Clostridium acetobutylicum在進行ABE發酵時，若發酵液中丁醇抑制物濃度達到10 g/L就會對菌體產生嚴重的抑制影響而失活，因此藉由疏水性海綿透過萃取式發酵以降低丁醇帶來的抑制效應，並提升丁醇產量。
本實驗將探討一項新的製程，利用疏水性PDMS海綿作為抑制物萃取劑，在菌體發酵至72小時進行丁醇萃取，海綿萃取劑對發酵液重量百分比為3.5%，萃取時間為1.5小時。經過萃取後，丁醇濃度由6.23 g/L下降至5.03g/L，且在84小時達最高產量10.72 g/L。與控制組相比，丁醇總產量提升15.02%，丁醇濃度達最大值之發酵時間由96小時提前至84小時，雖然菌體在丁醇萃取後沒有增加的趨勢，但可減緩其失活速度，因此利用PDMS疏水海綿作為萃取劑之製程可行性，值得深入探討。

In this research, we use hydrophobic Polydimethylsiloxane(PDMS) sponge to extract butanol from fermentation of Clostridium acetobutylicum, then discuss its effect and feasibility. When the concentration of butanol get 10 g/L in ABE fermentation, the Clostridium acetobutylicum will be inhibited by the product, butanol, and lose activation. The extractive fermentation will improve this problem by reducing the concentration of butanol.
The experiment will explore a new process using PDMS sponge as the extractant. we extract by PDMS sponge (3.5wt%)in 1.5 hours when the fermentation gets to 72 hours, the concentration of butanol reduced from 6.23 g/L to 5.03 g/L. After 84 hours, the maximum yield of butanol is 10.72 g/L and higher than controlled experiment (9.32 g/L), and time of the butanol reach to maximum concentration is advanced from 96 to 84 hours compared to control experiment. The biomass of bacterium is no significant change after extracting, but the deactivation of bacterium is slow down. Therefore, the process feasibility of using PDMS hydrophobic sponge as extractant, is worth in-depth study.

摘要    i
Abstract    ii
目錄    iii
圖目錄    vi
表目錄    viii
第一章    緒論    1
1    研究動機    1
2    研究目的    2
第二章    文獻回顧    3
1    再生能源    3
1.1    再生能源介紹    3
1.2    生質能源發展及應用    4
1.3    生質丁醇    4
2    丁醇生產方法    5
3    ABE發酵    6
3.1    ABE發酵介紹    6
3.2    ABE發酵製程改良    7
4    丙酮丁醇梭菌介紹    9
5    聚二甲基矽氧烷(Polydimethylsiloxane)    14
6    吸附基本原理    16
6.1    吸附現象    16
6.2    吸附種類    18
第三章    材料與方法    20
1    實驗規劃    20
2    實驗材料    21
2.1    實驗菌株    21
2.2    實驗藥品    22
3    實驗方法    24
3.1    菌種保存    24
3.2    培養基組成    25
3.3    接種菌體培養    28
3.4    PDMS海綿製備    29
3.5    海綿吸收效率及吸附曲線測定    30
3.6    海綿對於菌體之毒性測試    32
3.7    萃取式發酵    32
4    分析方法    33
4.1    菌體量測定    33
4.2    葡萄糖殘量分析    34
4.3    ABE分析    35
第四章    實驗結果討論    37
1    PDMS海綿基本性質    37
1.1    海綿表面性質    37
1.2    海綿製備比例對於海綿穩定度之影響    38
1.3    海綿對於ABE溶劑之吸附曲線    39
1.4    海綿對於菌體之毒性測試    41
2    以PDMS海綿對ABE發酵進行萃取式發酵    43
2.1    控制組實驗    43
2.2    海綿萃取丁醇時間點對萃取式發酵之影響    44
第五章    結論與建議    48
1    結論    48
2    建議    49
第六章    參考文獻    50

                                

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